山东大学硕上学位论文
9．与对照组以及过表达dXbpIs组对比，过表达dFoxO组果蝇脂肪体内的脂滴 明显变小，同时过表达dXbpls导致脂滴大小恢复。
结论：
1．饥饿状态下，内质网应激反应的激活能够参与到脂肪动员过程中，果蝇脂肪 体内的内质网应激蛋白dXBPls能够通过减少脂肪的过度消耗延长果蝇的存 活时间。
2．果蝇脂肪体中的内质网应激蛋白dXBPl可能通过控制dFOXO蛋白的降解调 控果蝇幼虫的脂肪动员继而影响果蝇幼虫的发育。
3．饥饿条件下，果蝇脂肪体内的内质网应激蛋白dXBPl对dFOXO蛋白负调控 的机制是促进dFOXO蛋白的降解，且这种降解过程主要依赖于蛋白酶体而 不是胰岛素途径。更具体的作用机制仍有待进一步研究。
关键词：内质网应激；XBPl；果蝇；饥饿；FOXO
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万方数据
山东大学硕_上学位论文
stress function of reticulum The signaling protein endoplasmic mechanism Graduate in starved and the XBPI possible Drosophila Cui student：Shang Major：Cell Biology Mentor：Xiangdong Wang Co—mentor：Yong Liu
ABSTRACT
Endoplasmic reticulum(ER)exists in all the eukaryotic cells，which is the essential organelle that controls the synthesis，modification，processing and transmembrane proteins．When the newly synthesized transporting of secreting and to process or the cell encounters a unfavorable peptides exceed the capability of the ER condition such as Ca2+loss from ER to cytosol，the unfolded or misfolded proteins will accumulate in the ER will be forced to a stressful state，lumen，therefore，the ER stress．Once ER．stress OCCURS，the ER stress sensors on the ER which is defined as ER membrane will activate a series of signaling pathways called
unfolded protein response(UPR)to enhance protein processing capacity in ER or to
reduce the synthesis of to process and to attenuate stress． IRElproteins for ER finally 一XBP1 is the most conservative one in UPR previous reaction．Some signaling pathway data in our group showed I-XBP 1 signaling that，in mouse liver,the IRE pathway
is activated under starvation condition，and it regulates the metabolism adaptation response incurred by starvation via modulating the expression of(PPAR activated this as thesis，we llt(proliferation receptor alpha)．In exploited drosophila model animals，and focused the experiments on its fat body,which is the organ metabolism．Our results indicated that the ER closely related to energy storage and in stress XBPl able to reduce the of protein drosophila(dXBPl)is consumption fat， and extends the life span of Drosophila under starvation condition via enhancing the degradation of Forkhead box O protein in drosophila(dFOXO)．Our results also showed that the process of degradation is proteasome-dependent rather than insulin-
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万方数据
内质网应激蛋白Xbp1在果蝇饥饿过程中的功能及其作用机制研究-细胞生物学专业毕业论文
