基于转录组测序的黄麻SSR分子标记开发与初步验证
陶爱芬;陈婉婷;祁建民;徐建堂;林荔辉;林培清;张立武;方平平
【期刊名称】《中国农业大学学报》 【年(卷),期】2024(023)006
【摘要】In order to develop SSR markers and provide powerful tools for the molecular marker-assisted breeding of jute, the SSR loci from transcriptome
sequences
were
searched
by
MicroSAtellite
(MISA)software, and the polymorphism of some SSRs were identified. The result showed that:1)A total of 8 571 locus, which were longer than 1 kb and accounted for 62. 47％of the screened Unigenes, were identified from 13 718 Unigenes, resulting in one SSR per 3. 47 kb on average;2)Mononucleotide repeat was the most abundant repeat type, accounting for 42. 15％ of the total number of SSRs. The dinucleotide and trinucleotide repeat were less abundant, which ratio was 14. 80％ and16. 74％, respectively;3)A total of 880 primers related to lignin synthase gene and MYB transcription factor were designed by Primer 3. 0, among which 29 were screened and 8 pairs of primers produced polymorphic bands. The ratio of polymorphic primers was 27. 58％. In conclusion, it is feasible to develop SSR molecular markers based on transcriptome sequencing analysis of jute, which could be applied for analyzing genetic diversity, genetic linkage mapping and functional gene mining of jute.%为开发黄麻SSR引物,为黄麻分子标记辅助育种等研究
基于转录组测序的黄麻SSR分子标记开发与初步验证
