Comparative transcriptomic analysis of larval and adult Malpighian tubules from the cotton

Comparative transcriptomic analysis of larval and

adult Malpighian tubules from the cotton

bollworm Helicoverpa armigera

Yi-Yang Yuan;Mei Li;Fan Fan;Xing-Hui Qiu

【期刊名称】《中国昆虫科学：英文版》 【年(卷),期】2024(025)006

【摘要】Malpighian tubules (MTs) are usually considered the key excretory and osmoregulatory organs of insects.However,increasing evidence has suggested that MTs perform many more functions than just osmoregulation.Until now,the molecular and physiological functions of MTs in the cotton bollworm (Helicoverpa armigera),a very important agricultural pest,are largely unknown.In this study,the transcriptomes of H.armigera MTs from larvae,male adults and female adults were sequenced using RNA-Seq technology,and comparative analyses of transcriptomes between two life stages (larval and adult) and between adult sexes were conducted.We generated a total of 84 643 high-quality unigenes,and identified a large number of abundant transcripts

putatively

encoding

proteins

involved

in and

diuresis,detoxification,immunity,carbohydrate transport

metabolism,development and reproduction.We found that the expression pattern of unigenes was relatively similar between female and male adult MTs,but different between larval and adult MTs.Our data