闭花耳草的化学成分研究
徐 丽1，黎 平1，唐贵华2，吴世标3，王业玲1，Edward KENNELLY3，龙春林1，4*
【摘 要】摘 要:从闭花耳草(Hedyotis cryptantha)全草中分离得到11 个化合物，经波谱数据分析鉴定为车叶草苷(1)，车叶草苷酸(2)，车叶草苷酸甲酯(3)，车叶草酸乙酯(4)，3，4-二氢-3-甲氧基车叶草苷(5)，山奈酚(6)，kaempferol 3，7-di-O-β-D-glucoside(7)，kaempferol 3-O-β-D-galactopyranosyl-(1→3)-β-D-galactopyranoside(8)，乌索酸(9)，豆甾醇(10)，β-谷甾醇(11)。所有化合物均为首次从闭花耳草植物中分离得到，其中化合物7 和8 首次从耳草属(Hedyotis)植物中分离得到。 【期刊名称】广西植物 【年(卷),期】2014(000)006 【总页数】7
【关键词】关键词:闭花耳草;环烯醚萜;黄酮;三萜;甾醇
The Hedyotis(Rubiaceae)，a large genus(ca.699 species)of herbs or somewhat shrubby plants distributed throughout the tropics and subtropics and with 62 species and 7 varieties found in China(38 endemic)，has been widely used as a traditional medicine in a number of Asian countries(Wang et al.，2001;Ahmad et al.，2005).Various species of Hedyotis were known to have a range of important pharmacological effects such as anticancer，antimicrobial，anti-inflammatory，antiulcer and immunomodulating activities，and used
to treat various diseases including snakebites，sphagitis，bronchitis，hepatitis，pneumonia in children，appendicitis，pelvitis，tonsillitis，nephritis，rheumatic arthritis，infectious fever，dysentery，and some tumors in liver，lung and stomach(Peng et al.，1998;Zhang et al.，2010，2011).Previous chemical investigations of this genus have led to the isolation of a series of iridoid glycosides(Peng et al.，1999;Jiangsu Provincial Institute of Botany，1991)，triterpenoids(Si et al.，2010;Masatak et al.，1998;Hui et al.，1977)，flavonoids(Kim et al.，2001;Ju，2007)，anthraquinones(Shi et al.，2008;Yu et al.，2009;Rohaya et al.，2005)，phenolic glycosides(Wang et al.，2013)and alkaloids(Peng et al.，1997;Phuong et al.，1999)，and some of these compounds have significant bioactivities.
Hedyotis cryptantha is endemic to Hainan Island，China，which has been used as a traditional medicine by Li ethnic group for the treatment of injury.It was firstly reported in ethnobotanical investigation in Hainan Province(Zheng et al.，2009a，b).To the best of our knowledge，there is little phytochemical study on this plant.The present work is the first detailed phytochemical study on the species H.cryptantha.In total 11 compounds were isolated from the whole plant:asperuloside(1)，asperulosidic acid(2)，asperulosidic acid methyl ester(3)，asperulosidic acid ethyl ester(4)，3，4-dihydro-3-methoxy asperuloside(5)，kaempferol(6)，kaempferol 3，7-di-O-β-Dglucoside(7)，kaempferol3-
O-β-D-galactopyranosyl-(1→3)-β-d-galactopyranoside(8)acid(9)，stigmasterol(10)，and β-sitosterol(11).
，ursonic
1 Material and Method
1.1 Plant Material
The whole plants of H.cryptantha were collected from Lingshui County，Hainan，China in August 2010 and identified by one of our authors(Prof.Chun-Lin Long).The voucher specimen(No.20100814)was deposited at the Herbarium of Minzu University of China. 1.2 Experimental Instruments
1H and13C NMR spectra were recorded on Bruker AM-600 and Bruker DRX-500 spectrometers using TMS as an internal standard.HR-ESI-MS analyses were carried out on an LCT premier XE TOF mass spectrometer(Waters，Manifold，MA)instrument.Silica gel(80-100 and 300-400 mesh，Yantai Chemical Industry Institute Co.，Ltd.，China)，MCI gel CHP 20P(75-150 μm，Mitsubishi Chemical Corporation，Tokyo，Japan)，HSCCC(TBE-20A and TBE-300B，Shanghai Tauto Biotechnique，Shanghai，China)，Rp-C1 8(20-45 μm，BUCHI Labortechnic AG，Switzerland)and SephadexLH-20(GE Healthcare Bio-Xciences AB)were used for column chromatography，and silica gel GF254(Yantai)was used for preparative TLC in the form of precoated plates.TLC spots were visualized under UV light and by dipping into 5% H2SO4 in EtOH followed by heating.
闭花耳草的化学成分研究
