Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without pro

Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without

proteolytic activity

Jing Qian;Zhanyun Guo;Chengwu Chi

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2008(040)002

【摘要】We cloned and isolated a cysteine-rich protein, designated Mr30, from Conus marmoreus. Mr30 belongs to the cysteinerich secretory protein family that is highly homologous to Tex31 previously obtained from Conus textile and reported as a protease responsible for processing of pro-conotoxins. Mr30, purified by a procedure similar to that of Tex31,indeed showed low proteolytic activity. However, further investigations revealed that the detected protease activity actually resulted from a trace amount of protease(s) contamination rather than from Mr30 itself. This finding led us to rethink the role of conus cysteine-rich secretory proteins: they were probably not responsible for the processing of pro-conotoxins as previously deduced, but their real biological functions remained to be clarified. 【总页数】8页(174-181)

【关键词】cone snail; cysteine-rich secretory protein;protease; protease inhibitor; covalent modification

【作者】Jing Qian;Zhanyun Guo;Chengwu Chi

【作者单位】Institute of Protein Research, Tongji University, Shanghai 200092, China;Institute of Protein Research, Tongji University, Shanghai 200092, China;Institute of Protein Research, Tongji University, Shanghai 200092, China;Institute of Biochemistry and Cell Biology, Shanghai Institutes

for

Biological

Sciences,

Chinese

Academy

of

Sciences,rnShanghai 200031, China 【正文语种】中文 【中图分类】Q5 【相关文献】

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