Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus

Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus

Yuling Ding; Chanipa Jiratchayamaethasakul; Junseong Kim; Eun-A Kim; Soo-Jin Heo; Seung-HongLee

【期刊名称】《亚太热带生物医学杂志：英文版》 【年(卷),期】2020(000)001

【摘要】Objective:To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber,Stichopus

japonicus,collected

from

Jeju

Island.Methods:Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes(HaCaT)to determine its radical scavenging activity and

protective

effects

against

2,2’-azobis(2-oxidative

amidinopropane)dihydrochloride(AAPH)-induced

stress.Antimelanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and

mushroom

tyrosinase.Following

the

induction

byα-

melanocytestimulating hormone,the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity,melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor,tyrosinase,and tyrosinase-related proteins(TRP-1,and TRP-2)were examined.Results:The ultrasonic extract of red sea