Hypertonic saline resuscitation contributes to early accumulation of circulating myeloid-d

Hypertonic saline resuscitation contributes to early

accumulation of circulating myeloid-derived suppressor cells in a rat model of hemorrhagic

shock

LU Yuan-qiang;GU Lin-hui;ZHANG Qin;JIANG Jiu-kun;MOU Han-zhou

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2013(000)007

【摘要】Background Hemorrhagic shock is usually associated with complicated immune and inflammatory responses,which are sometimes crucial for the prognosis.As regulators of the immune and inflammatory system; proliferation,migration,distribution and activation of myeloid-derived suppressor cells (MDSCs) are intimately linked to the inflammation cascade.Methods In a model of severe hemorrhagic shock,thirty-five rats were randomly divided into control,sham,normal saline resuscitation (NS),hypertonic saline resuscitation (HTS),and hydroxyethyl starch resuscitation (HES),with seven in each group.M DSCs were analyzed by flow cytometric staining of CD11b/c+Gra+ in peripheral blood mononuclear cells (PBMC),spleen cell suspensions,and bone marrow nucleated cells (BMNC).Simultaneously,the expressions of arginase-1 (ARG-1) and inducible nitric oxide synthase (iNOS) mRNA in MDSCs were evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).Results In the early stage after hemorrhagic