香烟烟雾对大鼠脑血管细胞粘附分子-1表达的影响
孙芸;柳忠兰
【期刊名称】《中国现代医学杂志》 【年(卷),期】2006(016)001
【摘要】目的为研究香烟烟雾对大鼠脑血管细胞粘附分子(VCAM-1)表达的影响,探讨吸烟致脑梗死的一种分子机制.方法建立大鼠吸烟模型,免疫组化方法检测大鼠脑血管VCAM-1表达.结果吸烟大鼠脑血管VCAM-1表达增加(大量吸烟组平均灰度116.08±2.38,小量吸烟组123.06±1.68,对照组130.33±1.88,即大量吸烟组血管内皮染色最深,VCAM-1表达最强,小量吸烟组次之,对照组最弱.经统计学分析,三组数值之间两两比较,差异均有显著性意义,P＜0.05).结论香烟烟雾使大鼠脑血管VCAM-1表达增加,脑血管内皮细胞VCAM-1表达增强可能是吸烟引起脑梗死的分子机制之一.%[Objective] To study the effect of cigarette smoking on expression of VCAM-1 on cerebrovascular endothelium in rats for investigating one of molecular mechanisms of cerebral infarction induced by smoking. [Methods] Laboratory animals were grouped randomly into large-dose smoking, small-dose smoking and normal control.Then rats smoking models were made and immunohistochemical methods were used to detect the expression of VCAM-1 on cerebrovascular endothelial cells in smoking rats. [Results] The cerebrovascular endothelial cells were all expressed with positive signal (brown granules) in all three groups, which was to say they were all expressed with VCAM-1 protein. By one way analysis of variance, two
of three groups have marked statistical difference (P ＜0.05)separately on average gray value of VCAM-1 staining in vascular cellular endo the lium. And the mean gray value between large-dose and small-dose also included statistical difference. [Conclusions] Cigarette smoking can increase the expression of VCAM-1 in cerebrovascular endothelial cell; increasing expression of VCAM-1 in cerebrovascular cell may be one of the molecular mechanisms of cerebral infarction caused by smoking. 【总页数】4页(5-7,13)
【关键词】吸烟;脑血管内皮细胞;血管细胞粘附分子-1;大鼠 【作者】孙芸;柳忠兰
【作者单位】中国医科大学附属第一医院,辽宁,沈阳,110001;中国医科大学附属第一医院,辽宁,沈阳,110001 【正文语种】中文 【中图分类】R392.11 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_china-journal-modern-medicine_thesis/0201253982166.html 【相关文献】
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香烟烟雾对大鼠脑血管细胞粘附分子-1表达的影响
