不同类胡萝卜素清除自由基能力的比较

不同类胡萝卜素清除自由基能力的比较

The Comparison of Free Radical Scavenging Capacity of Carotenoids

袁磊;刘晓庚;唐瑜;

? ?

1:南京财经大学食品科学与工程学院 2:江苏省粮油品控重点实验室

摘要： 比较不同结构类胡萝卜素清除自由基能力。分别以虾青素、β-胡萝卜素、番茄红素和叶黄素为对象,采用DPPH法、水杨酸法和邻苯三酚自氧法分别表征清除DPPH·自由基、羟

基自由基和超氧阴离子自由基能力。结果表明类胡萝卜素具有较强的清除自由基能力,且清除能力均遵循剂量-效应关系;对DPPH·自由基、羟基自由基和超氧阴离子的清除能力大小关系为虾青素>叶黄素>β-胡萝卜素>番茄红素。类胡萝卜素清除自由基能力优良,不同结构类胡萝卜素清除自由基能力有差异,此类功能性产品具有广阔的市场前景。

关键词： 类胡萝卜素;;结构;;自由基;;清除能力

Abstract: Keywords: 基金项目: 江苏省粮油品质控制及深加工技术重点实验室资助项目(LYPK201301)

作者: 袁磊;刘晓庚;唐瑜;

Email:

参考文献：

? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

[1]Shon M Y,Kim T H,Sung N J.Antioxidants and free radical scavenging activity of Phellinus baumii(Phellinus of Hymenochaetaceae)extracts[J].Food Chemistry,2003,82(4):593-597.

[2]方允中,杨胜,伍国耀.自由基、抗氧化剂、营养素与健康的关系[J].营养学报,2003,25(4):337-343.

[3]Rao P S,Kalva S,Yerramilli A,et al.Free Radicals and Tissue Damage:Role of Antioxidants[J].Free Radicals and Antioxidants,2011,4:2-7.

[4]Fujisawa S,Atsumi T,Kadoma Y,et al.Antioxidant and prooxidant action of eugenol-related compounds and their cytotoxicity[J].Toxicology,2002,177:39-54. [5]Michaud D S,Feskanich D,Rimn E B,et al.Intake of specific carotenoids and risk of lung cancer in 2 prospective US cohorts[J].Journal of Clinical Nutrition,2000,72(4):990-997.

[6]Bendich A.Carotenoids and the Immune Response[J].The Journal of Nutrition,1989,119(1):112-115. [7]范晓岚,杨军,糜漫天,等.β-胡萝卜素的抗氧化作用与疾病预防[J].中国公共卫生,2003,19(4):479-480. [8]孙震,姚惠源.类胡萝卜素的抗氧化活性与其量子化学参数的相关研究[J].食品与机械,2007,23(4):56-59. [9]宋素梅.南极磷虾中虾青素的提取与分离纯化[D].无锡:江南大学,2013.

[10]胡小明,蔡万玲,代斌.天然β-胡萝卜素的提取工艺条件研究[J].食品工业科技,2006,27(10):133-136. [11]赵功玲.番茄红素的提取及纯化工艺研究[D].杨凌:西北农林科技大学,2005.

[12]Mellors A,Tappel A L.The inhibition of mitochondrial peroxidation by ubiquinone and ubiquinol[J].Journal of Biologieal Chemistry,1966,241(19):4353-4356. [13]Rufian H J A,Morales N F J.Effect of in vitro enzymatic digestion on antioxidant activity of coffee melanoidins and fractions[J].Journal of Agricultural and Food Chemistry,2007,55(24):10016-10021.

[14]王建华,张民,甘璐,等.枸杞多糖-2的抗羟基自由基氧化作用[J].食品科学,2001,22(1):11-13. [15]吴亚楠,鲁晓翔,连喜军.玉米须黄酮清除自由基活性的研究[J].食品研究与开发,2009,30(1):5-8.

[16]陈留勇,孟宪钧,贾薇,等.黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究[J].食品科学,2004,24(1):167-170. [17]许申鸿,杭瑚,李运平.超氧化物歧化酶邻苯三酚测活法的研究及改进[J].化学通报,2001,64(8):516-519.

[18]Smoluk G D,Fahey R C,Ward J F,et al.Interaction of glutathione and other low-molecular weight thiols with DNA:Evidence for counter ion condensation and colon depletion near DNA[J].Radiation Research,1998,114(1):3-10.

[19]王云海,罗云敬,钟儒刚.过氧亚硝酸根对蛋白质损伤的研究进展[J].化学进展,2007,19(6):893-901.

? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

[20]Shen H M,Yang F C,Ding W X,et al.Superoxide radical-initiated apoptotic signaling pathway in selenite-treated Hep G(2)cells:mitochonaria serve as the main target[J].Free Radical Biology&Medicine,2001,30(1):9-21.

[21]Hosaka S,Obuki M,Nakajima J,et al.Comparative study of antioxidant as quenchers or scavengers of reactive oxygen species based on quenching of MCLA-dependent chemiluminescence[J].Luminescence,2005,20(6):419-427.

[22]Sumanont Y,Murakami Y,Tohda M,et al.Evaluation of the nitric oxide radical scavenging activity of manganese complexes of curcumin and its derivative[J].Biological&Pharmaceutical Bulletin,2004,27(2):170-173.

[23]Britton G.Structure and properties of carotenoids in relation to function[J].The Journal of the Federation of American Societies for Experimental Biology,1995,9(15):1551-1558.

[24]周敏娜,杨英杰,吕庆章.4种类胡萝卜素抗氧化性质的量化计算[J].计算机与应用化学,2011,28(10):1305-1308. [25]陈东方,王海玉,李立,等.天然虾青素缓解体力疲劳作用的实验研究[J].中国现代医学杂志,2010,20(17):2567-2569. [26]陈志强,任璐,江慎华,等.虾青素降血糖作用的研究[J].食品科学,2008,29(7):402-404.

[27]Gunasekera R S,Sewgobind K,Desai S,et al.Lycopene and lutein inhibit proliferation in rat prostate carcinoma cell[J].Nutrition and Cancer,2007,58(2):171-177. [28]Olmedilla B,Granado F,Blanco I,et al.Lutein,but notα-tocopherol,supplementation improves visual function in patients with age-related cataracts:A 2-y doubleblind,placebo-controlled piot study[J].Nutrition,2003,19(1):21-24.

[29]Bertram J S,Pung A,Churley M,et al.Diverse carotenoids protect against chemically induced neoplastic transformation[J].Carcinogenesis,1991,12(4):671-676. [30]Fryburg D A,Mark R J,Griffith B P,et al.The effect of supplemental beta-carotene on immunologic indices in patients with AIDS:a pilot study[J].The Yale Journal of Biology and Medicine,1995,68(1):1923.

[31]Blum A,Monir M,Wirsansky I,et al.The beneficial effects of tomatoes[J].European Journal of Internal Medicine,2005,16(6):402-404.

[32]Rao L G,Mackinnon E S,Josse R G,et al.Lycopene consumption decreases oxidative stress and bone resorption markers in postmenopausal women[J].Osteoporosis International,2007,18(1):109-115.

[33]赵娟娟.番茄红素的抗氧化活性研究[J].食品科技,2010,35(7):62-65. [34]赵学超.蛋黄中叶黄素分离纯化及功能性研究[D].无锡:江南大学,2009.

[35]李大婧,刘志凌,刘春泉.叶黄素酯和叶黄素抗氧化作用比较[J].中国食品学报,2008,8(5):28-32.