Downregulation of Notch-regulated Ankyrin Repeat Protein Exerts Antitumor Activities again

Downregulation of Notch-regulated Ankyrin Repeat Protein Exerts Antitumor Activities against

Growth of Thyroid Cancer

Bing-Feng Chu;Yi-Yu Qin;Sheng-Lai Zhang;Zhi-Wei Quan;Ming-Di Zhang;Jian-Wei Bi

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2016(129)013

【摘要】Background:The Notch-regulated ankyrin repeat protein (NRARP) is recently found to promote proliferation of breast cancer cells.The role of NRARP in carcinogenesis deserves extensive investigations.This study attempted to investigate the expression of NRARP in thyroid cancer tissues and assess the influence of NRARP on cell

proliferation,apoptosis,cell

cycle,and

invasion

in

thyroid

cancer.Methods:Thirty-four cases with thyroid cancer were collected from the Department of General Surgery,Xinhua Hospital,Shanghai Jiao Tong

University

School

of

Medicine

between

2011

and

2012.Immunohistochemistry was used to detect the level of NRARP in cancer tissues.Lentivirus carrying NRARP-shRNA (Lenti-NRARP-shRNA) was applied to down-regulate NRARP expression.Cell viability was tested after treatment with Lenti-NRARP-shRNA using 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide assay.Apoptosis and cell cycle distribution were determined by flow cytometry.Cell invasion

was

tested

using

Transwell

invasion

assay.In