Carbon monoxide decreases interleukin-1β levels in the lung through the induction of pyrin

Carbon monoxide decreases interleukin-1β levels

in the lung through the induction of pyrin

Seul-Ki Kim;Yeonsoo Joe;Yingqing Chen;Jinhyun Ryu;Jeong-Hee Lee;Gyeong Jae Cho;Stefan W.Ryter;Hun Taeg Chung

【期刊名称】《中国免疫学杂志（英文版）》 【年(卷),期】2017(014)004

【摘要】Carbon monoxide (CO) can act as an anti-inflammatory effector in mouse models of lung injury and disease,through the downregulation of pro-inflammatory cytokines production,though the underlying

mechanisms

remain

unclear.The

nucleotide-binding

oligomerization domain-,leucine-rich region-,and pyrin domain-containing-3 (NLRP3) inflammasome is a protein complex that regulates the maturation and secretion of pro-inflammatory cytokines,including interleukin-1β (IL-1β).In this report,we show that the CO-releasing molecule (CORM-2) can stimulate the expression of pyrin,a negative regulator of the NLRP3 inflammasome.CORM-2 increased the transcription of pyrin in the human leukemic cell line (THP-1) in the absence and presence of lipopolysaccharide (LPS).In THP-1 cells,CORM-2 treatment dose-dependently reduced the activation of caspase-1 and the secretion of IL-1β,and increased the levels of IL-10,in response to LPS and adenosine 5'-triphosphate (ATP),an NLRP3 inflammasome activation model.Genetic interference of IL-10 by small interfering RNA