瞬时受体电位M7通道蛋白对大鼠肝星状细胞凋亡的影响
刘红;李俊;黄成;黄艳;周德喜
【期刊名称】《中国药理学通报》 【年(卷),期】2012(028)004
【摘要】Aim To investigate the effects of downex-pression of transient receptor potential melastatin 7 (TRPM7 ) and its internal mechanisms on apoptosis through transfecting specific siRNA in rat hepatic stellate cell lines HSC-T6. Methods Specific TRPM7-siRNA was transfected by Lipofectamine? 2000. Cell apoptosis was measured by Flow Cytometry. TRPM7 and Caspase-3 protein expression were analyzed by Western blot; TRPM7, Caspase-3 and Bid mRNA expression were evaluated by RT-PCR. Results Sup-pression TRPM7 expression by siRNA significantly increased the apoptosis of HSCs, the expression of Caspase-3 protein and Bid mRNA compared with normal or control. Conclusions Specific TRPM7-siRNA not only inhibits the expression of TRPM7 but also induces apoptosis of HSCs. The enhanced gene expression of Bid may contribute to HSCs apoptosis.%目的 通过转染特异性siRNA,下调瞬时受体电位M7通道蛋白(TRPM7)在大鼠肝星状细胞系HSC -T6中的表达,研究其对HSC-T6凋亡的影响及内在机制.方法 利用Li-pofectamineTM 2000将特异性TRPM7-siRNA转染到HSC-T6内,分别运用流式细胞术检测HSCs凋亡变化；Western blot 检测TRPM7、Caspase-3蛋白的表达；RT-PCR检测TRPM7,Caspase-3、Bid mRNA的表达.结果 TRPM7-siRNA明显抑制
瞬时受体电位M7通道蛋白对大鼠肝星状细胞凋亡的影响
