紫甘薯花色苷对膀胱癌BIU87细胞增殖的影响及其机制探
讨
李卫林;季广华;章新展;俞洪元
【期刊名称】《中华医学杂志》 【年(卷),期】2024(098)006
【摘要】Objective To observe the effect of purple sweet potato anthocyanins on the proliferation of bladder cancer cell line BIU87 and to investigate the molecular mechanisms.Methods Bladder cancer BIU87 cells were cultured and exposed to anthocyanins at the different concentrations of 100,200,400,and 800 μg/ml respectively.The growth inhibition of anthocyanins on BIU87 cells were evaluated by morphometry and cell counting kit-8 (CCK-8) assay,and the cell apoptosis rate was detected by Flow cytometry (FCM).Results Morphometry showed that the number of BIU87 cells decreased,the volume shrank,the intercellular space enlarged,the ability of cell adherence weakened,and the cell shape changed when the concentration of anthocyanins increased.CCK-8 assay showed that when 100,200,400,800 μg/ ml anthocyanins treated BIU87 cells for 48 h,the absorbance was 24 ± 0.07,1.15 ± 0.11,0.90 ± 0.08,0.56 ± 0.09,respectively.Compared with the control group,anthocyanins-treated groups significandy inhibited the proliferation of BIU87 cells (P ＜ 0.05).FCM test showed that after treatment with different doses of
紫甘薯花色苷对膀胱癌BIU87细胞增殖的影响及其机制探讨
