mTOR 信号通路与非小细胞肺癌放疗敏感性的关系
廖娟;梁静;雎岩;赵金;李洁;李涛;杨怡萍
【期刊名称】《现代肿瘤医学》 【年(卷),期】2015(000)018
【摘要】目的：研究 mTOR 信号通路与非小细胞肺癌放疗敏感性的关系。方法：采用不同剂量的雷帕霉素抑制非小细胞肺癌细胞系 A549中的 mTOR 信号通路；Western blot 检测雷帕霉素作用后下游信号分子的表达；将 A549细胞分为空白对照组及雷帕霉素作用的实验组，将对照组和实验组经不同剂量射线作用；用 MTT 实验（噻唑蓝）及克隆形成能力检测细胞增殖能力，流式细胞仪检测细胞凋亡的变化。结果：雷帕霉素药物作用抑制了 mTOR 信号通路及下游分子的表达，经不同剂量射线照射后细胞的增殖能力和克隆形成能力显著低于对照组，凋亡显著高于对照组。结论：抑制 mTOR 信号通路可以增加非小细胞肺癌对放疗的敏感性。%Objective:To investigate the correlation between the activation of mTOR signal pathway and the radio-sensitivity in non -small cell lung cancer (NSCLC).Methods:mTOR pathway was inhibited by different doses of Rapamycin in NSCLC cell line A549.Downstream signaling molecules were detected by Western blot.A549 was divid-ed into the control group and experimental group which was treated by rapamycin,following with different dose of radi-ation.The cell growth was evaluated by MTT assay and clone formation assay.Apoptosis was determined
by
FACS
(fluorescent
-activated
cell
sorting).Results:Rapamycin suppressed the mTOR signal pathway and
mTOR 信号通路与非小细胞肺癌放疗敏感性的关系
