Effect of Autophagy Inhibition on the Protection of Ischemia Preconditioning against Myoca

Effect of Autophagy Inhibition on the Protection of

Ischemia Preconditioning against Myocardial Ischemia/Reperfusion Injury in Diabetic Rats

Ya-Yang Liu;Chao Sun;Fu-Shan Xue;Gui-Zhen Yang;Hui-Xian Li;Qing Liu;Xu Liao

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2024(131)014

【摘要】Background:Ischemia preconditioning (IPC) remains the most powerful

intervention

of

protection

against

myocardial

ischemia/reperfusion injury (IRI),but diabetes can weaken or eliminate its cardioprotective effect and detailed mechanisms remain unclear.In this study,we aimed to explore whether changes of autophagy in the diabetic condition are attributable to the decreased cardioprotective effect of IPC.Methods:Sixty diabetic male Sprague-Dawley rats were randomly divided into the control (C),IRI,rapamycin (R),wortmannin (W),rapamycin + IPC (R + IPC),and wortmannin + IPC (W + IPC) groups.The in vivo rat model of myocardial IRI was established by ligaturing and opening the left anterior descending coronary artery via the left thoracotomy.Durations of ischemia and reperfusion are 30 min and 120 min,respectively.Blood samples were taken at 120 min of reperfusion for measuring serum concentrations of troponin Ⅰ (TnI) and creatine kinase isoenzyme MB (CK-MB) using the enzyme-linked immunosorbent assay.The infarct size was assessed by Evans blue and