胰液中K-ras基因突变和p16基因甲基化改变联合检测在
胰腺癌诊断中的初步研究
刘枫;李兆中;满晓华;许国铭;邹多武;方琳;周国雄
【期刊名称】《中华肝胆外科杂志》 【年(卷),期】2008(014)007
【摘要】Objective To investigate the diagnostic significance of detecting p16 aberrant meth-ylation and K-ras gene mutation in pancreatic juice for pancreatic cancer. Methods Aberrant p16 methylation was detected using the methylation-specific PCR (MSP) in pure pancreatic juice(PPJ) col-lected from the pancreatic duct through a nasopancreatic tube put under endoscopic retrograde cholan-giopancreatography (ERCP). K-ras gene 12 codon mutation was detected using the m PCR-RFLP in the pure PPJ. Results The DNA was obtained from PPJ through a nasopancreatic tube. Aberrant p16 methylation and K-ras gene mutation were detected in the same samples of PPJ. Sensitivity, specifici-ty,positive predictive values, negative predictive values and accuracy of HE staining for pancreatic cancer were 40%, 100%, 100%,45.4% and 60.0%, respectively. Of the 20 cases of pancreatic canc-er,K-ras gene mutation was detected in 14 (70%) and the p16 gene was shown to be methylated in 7(35%). Of the 8 cases of chronic pancreatitis,K-ras gene mutation was detected in 2 (25%). Of the 2cases of mucinous cystoadenoma of pancreas,K-ras gene mutation was
胰液中K-ras基因突变和p16基因甲基化改变联合检测在胰腺癌诊断中的
