Histological changes and antidiabetic activities of Icacina trichantha tuber extract in be

Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta-cells of

alloxan induced diabetic rats

Onakpa Michael Monday;Asuzu Isaac Uzoma

【期刊名称】《亚太热带生物医学杂志（英文版）》 【年(卷),期】2013(000)008

【摘要】Objective: To investigate the antidiabetic, hypolipidaemic activities and histopathological changes of Icacina trichantha (I. trichantha) tuber extract in alloxan induced diabetic rats.
Methods:In the present study, 80% methanol extract of I. trichantha tuber was tested on alloxan induced diabetic rats. They were randomly grouped into control (distilled water and glibenclamide) and experimental (200, 400 and 600 mg/kg body weight). Diabetes was induced by a single intraperitoneal injection of 160 mg/kg body weight of alloxan. Blood glucose levels were measured using blood glucose test strips with AccuCheck Advantage II glucometer at 1, 3, 6, and 24 h on the first day and 1 h after treatment on Day 7, 14 and 21. Blood samples were collected and centrifuged to separate serum for estimation of lipid profile and other biochemical parameters. Histopathological changes in diabetic rats pancreas were also studied after extract treatment.
Results: Daily oral administration of I. trichantha tuber extract (200, 400, and 600 mg/kg body weight) and glibenclamide (2 mg/kg) showed