Antioxidant, anti-quorum sensing and anti-biofilm potential of ethanolic leaf extract of P

Antioxidant, anti-quorum sensing and anti-biofilm potential of ethanolic leaf extract of Phrynium

capitatum and Dryptes indica

Nagaraju Jalli;Santhi Sri KV;Sairengpuii Hnamte;Subhaswaraj Pattnaik;Parasuraman Paramanantham;Busi Siddhardha

【期刊名称】《亚太热带生物医学杂志（英文版）》 【年(卷),期】2019(009)008

【摘要】Objective: To investigate the antioxidant and anti-infective potential of Phrynium capitatum and Dryptes indica extract. Methods: The antioxidant potentials were determined by DPPH radical scavenging, reducing power, hydroxyl radical scavenging and total antioxidant assays. We further examined anti-quorum sensing activity and inhibition of synthesis of pathogenic factor of Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Bioactive compounds were determined using gas chromatography–mass spectrometry analysis. In silico analysis was conducted to determine the binding affinity of bioactive compounds of plant extracts for the quorum sensing regulatory receptor LasR. Results: DPPH assay showed that the ethanolic extract of Phrynium capitatum and Dryptes indica at 500 μg/mL showed (86.96 ± 4.07)％ and (74.83 ± 3.47)％ inhibition, respectively. Hydroxyl radical scavenging assay showed (73.17 ± 3.03)％ and (62.63 ± 4.59)％ activity, respectively. The ethanolic extract of