补骨脂素与异补骨脂素对乳鼠颅骨成骨细胞分化成熟影响
的比较研究
翟远坤;潘亚磊;牛银波;武祥龙;梅其炳
【期刊名称】《中国药理学通报》 【年(卷),期】2012(028)003
【摘要】Aim To investigate the effects of psoralen and isopsoralen on the differentiation and maturation of rat calvarial osteoblasts ( ROB ). Methods Neonatal SD rat skull was segregated to obtain ROB with enzyme digestion. The culture medium was replaced every three days. Serial subcultivation proceeded when cells were covered with 80% culture dish. Psoralen and isopsoralen were supplemented into the culture at 1 × 10-5 mol · L-1 respectively. The osteogenic differentiation markers including alkaline phosphatase( ALP ) activity , CFU-FALP, osteocalcin secretion, calcium deposi-tion and mineralized bone modulus were compared a-mong the psoralen-supplemented group, the isopsoralen group and the control group. The gene expression of IGF-1, Osterix, Runx-2 and collagen I was examined by Real Time RT-PCR. Total protein was isolated and the secretion of collagen I was examined by Western blot. Results Both psoralen and isopsoralen significantly improved ALP activity, CFU-FALP amount, osteocalcin secretion, calcium deposition and mineralized modulus. Besides, they enhanced the mRNA level of IGF-1, Osterix , Runx-2 and collagen I , the
补骨脂素与异补骨脂素对乳鼠颅骨成骨细胞分化成熟影响的比较研究
