缬沙坦与螺内酯对肾血管性高血压大鼠心肌中TGF-β1表
达的影响
王圣;刘宇飞;曲鹏
【期刊名称】《海南医学》 【年(卷),期】2011(022)003
【摘要】Objective To assess the roles of valsartan and spironolactone on the myocardial fibrosis and the expression of TGF-β1 during the development of left ventricular hypertrophy (LVH) in renovascular hypertension rats.Methods Two-kidney, one-clip (2K1C) renovascular hypertension was induced in Sprague-Dawley rats. The rats were randomized
into
untreated
hypertension
group
(H
group),
spironolactone group (spironolactone 50 mg . kg-l·d-1,S group), valsartan group (valsartan 30 mg . kg-l·d-1, V group), and spironolactone plus valsartan group (spironolactone 50 mg·kg-l· d-l and valsartan 30 mg·kg-l · d-l at the same time, S+V group). Sham-operated rats served as controls (C group). The changes of heart structure and function in all groups were observed with echocardiogram. Left ventricle concentration of angiotensin Ⅱ (Ang Ⅱ) and ALD was assessed by radioimmunoassay. The Woessner' s method was used to estimate the collagen content in left ventricle. Immunohistochemistry was adopted to examine the protein expression of TGF-β1. Results After 8 weeks treatment, carotid systolic pressure, MESS (meridian end
缬沙坦与螺内酯对肾血管性高血压大鼠心肌中TGF-β1表达的影响
