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PROTEASE Thrombin Leu-Val-Pro-Arg??Gly-Ser Factor Xa Ile-Glu/Asp-Gly-Arg?? Enterokinase Asp-Asp-Asp-Asp-Lys?? TEV protease Glu-Asn-Leu-Tyr-Phe-Gln??Gly PreScission Leu-Glu-Val-Leu-Phe-Gln??Gly-Pro TAGZyme His-tag removal by Exoproteolytic Digestion Intein Site dithiothreitol cleavage HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln??Gly-Pro SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO COMPANY Amersham-Biosciences, Novagen, SIGMA, Roche Amersham-Biosciences, New England Biolabs, Roche New England Biolabs, Novagen, Roche Protease Capture Benzamidine-Agarose Benzamidine-Agarose Trypsin Inhibitor-Agarose Invitrogen – Life Technologies Ni-NTA (6His recomb. TEV) Amersham-Biosciences GSTrap for GST fusion enzyme Ni-NTA (6His recomb. enzyme) DTT elimination by dialysis Ni-NTA (6His recomb. enzyme) Qiagen New England Biolabs Novagen Invitrogen – Life Technologies, LifeSensors Ni-NTA (6His recomb. enzyme)
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