各种限制性酶的酶切位点
Enzyme Name Sequence
AatI AGG!CCT AatII GACGT!C AccI GT!MKAC AccII CG!CG AccIII T!CCGGA AcyI GR!CGYC AflI G!GWCC AflII C!TTAAG AflIII A!CRYGT AhaI CC!SGG AhaII GR!CGYC AhaIII TTT!AAA AluI AG!CT AlwI GGATCNNNN! AlwI !NNNNNGATCC AlwNI CAGNNN!CTG AocI CC!TNAGG AocII GDGCH!C AosI TGC!GCA AosII GR!CGYC
ApaI GGGCC!C ApaLI G!TGCAC ApyI CC!WGG AquI C!YCGRG AseI AT!TAAT AspI GAANN!NNTTC AspI G!GTACC AspI GACN!NNGTC AsuI G!GNCC AsuII TT!CGAA AvaI C!YCGRG AvaII G!GWCC AvaIII ATGCA!T AvrI !CYCGRG AvrII C!CTAGG AxyI CC!TNAGG BalI TGG!CCA BamHI G!GATCC BanI G!GYRCC BanII GRGCY!C BanIII AT!CGAT BbeI GGCGC!C
BbiII/AcyI GR!CGYC BbvI GCAGCNNNNNNNN! BbvI !NNNNNNNNNNNNGCTGC BbvII GAAGACNN! BbvII !NNNNNNGTCTTC BcefI ACGGCNNNNNNNNNNNN! BcefI !NNNNNNNNNNNNNGCCGT BclI T!GATCA BcnI CC!SGG BglI GCCNNNN!NGGC BglII A!GATCT BinI !NNNNNGATCC BinI GGATCNNNN! BsePI !GCGCGC BsmAI !GTCTC BsmAI !GAGAC BsmI GAATGCN! BsmI G!CATTC BspI GDGCH!C BspHI T!CATGA BspMI ACCTGCNNNN! BspMI !NNNNNNNNGCAGGT
BspMII T!CCGGA BsrI ACTGGN! BsrI C!CAGT BssHII G!CGCGC BstBI TT!CGAA BstEII G!GTNACC BstI G!GATCC BstNI CC!WGG BstPI G!GTNACC BstUI CG!CG BstXI CCANNNNN!NTGG BstYI R!GATCY BsuI CC!TNAGG CcrI C!TCGAG CfoI GCG!C CfrI R!CCGGY CfrI G!GNCC CfrI Y!GGCCR ClaI AT!CGAT CviJI RG!CY CvnI CC!TNAGG DdeI C!TNAG
DpnI GA!TC DraI TTT!AAA DraII RG!GNCCY DraIII CACNNN!GTG DsaI C!CRYGG EaeI Y!GGCCR EagI C!GGCCG EarI !CTCTTC EarI !GAAGAG EclXI C!GGCCG EcoI TAC!GTA EcoI GGTCTCN! EcoI !NNNNNGAGACC EcoI G!GWCC EcoIII AGC!GCT EcoI C!GGCCG EcoI !CTGAAG EcoI !CTTCAG EcoI CC!TNAGG EcoNI CCTNN!NNNAGG EcoOI RG!GNCCY EcoRI G!AATTC