α-Glucosidase Inhibitory Activities of Lutein and Zeaxanthin Purified from Green Alga Chl

α-Glucosidase Inhibitory Activities of Lutein and Zeaxanthin Purified from Green Alga Chlorella

ellipsoidea

QI Jia;KIM Sang Moo

【期刊名称】《中国海洋大学学报（英文版）》 【年(卷),期】2018(017)004

【摘要】α-Glucosidase inhibitors are used therapeutically to treat type-2 diabetes

mellitus.Through

a

bioassay-guided

fractionation

technique,three carotenoids,(all-E)-lutein,(all-E)-zeaxanthin and (9-Z)-zeaxanthin,were purified from the green alga Chlorella ellipsoidea,in which (all-E)-lutein and (9-Z)-zeaxanthin had potent α-glucosidase inhibitory activity.IC50 values of (all-E)-lutein and (9-Z)-zeaxanthin were 70 and 53.5 μmol L-1 against Saccharomyces cerevisiae α-glucosidase,respectively,with addition,IC50

values

of

non-competitive (9-Z)-zeaxanthin

inhibition.In

against

Bacillus

stearothermophilus and rat-intestinal α-glucosidase were 805.1 and 671.2 μtmol L-1,respectively.The Ki values of (all-E)-lutein and (9-Z)-zeaxanthin against S.cerevisiae α-glucosidase were 78.1 and 16.5 μmol L-1,respectively.Therefore,C.ellipsoidea carotenoids might be utilized as a novel candidate to prevent type-2 diabetes mellitus related disorders in food and medical industry. 【总页数】7页(983-989)