环孢素A对大鼠缺血再灌注心肌嘌呤代谢的影响
甄江涛;樊燕燕;赵晓丽;王丽艳;曹颖;于公元;康英姿
【期刊名称】《天津医科大学学报》 【年(卷),期】2012(018)001
【摘要】Objective: To study the effect of ciclosporin A (CsA) on purine metabolism in rats with rayocardial ischemia-reperfusion injury and its possible mechanism of myocardial protective effect. Methods: Myocardial ischemia-reperfusion models of 48 rat hearts was established with Langendorff model. 48 hearts were randomly divided into control group, reperfused group and CsA group. Control group were perfused with K-B solution for 90 min. Reperfused group were subjected to ischemia and reperfusion after 30 min perfusion with K-B solution. CsA group were also subjected to ischemia and reperfusion after 30 min perfusion, but CsA 0.2μmol/L were added into the perfusate during the later 20 min perfusion. The purine releases in the coronary artery effluent after the whole process and the activities of PNPase in the myocardial tissue were measured by HPLC. The activities of the effluent of coronary artery lactate dehydrogenase(LDH) and myocardial infarction size in myocardial tissue were assayed. Results: Ciclosporin A depressed the activity of coronary artery LDH and myocardial infarction size, and significantly increased the release of adenosine and inosine, decreased the release of uric acid after is-
环孢素A对大鼠缺血再灌注心肌嘌呤代谢的影响
