Animal study on CD4+ CD25+ regulatory T cells for treating female mouse with recurrent spo

Animal study on CD4+ CD25+ regulatory T cells for treating female mouse with recurrent spontaneous

abortion

Biao Duan;Lu Wang;Weiwei Huang;Shouhong Wang;Haiyan Du

【期刊名称】《临床病例讨论杂志（英文版）》 【年(卷),期】2015(002)004

【摘要】Objective: To explore immunotherapy effectiveness of the CD4+ CD25+ regulatory T cells for treating female mouse with recurrent spontaneous abortion (RSA) by animal experiments. Methods: Mononuclear lymphocytes were isolated from the blood (instead of cord blood) of new-born baby of KunMing Bai mouse or BALB/c male mouse with normal birth ability (as unrelated third party blood source) by density gradient centrifuga-tion method. The CD4+CD25+ regulatory T cells were selected by magnetic-activated cell sorting from mononuclear cells of cord blood cells. CBA/J female mouse copulated with DBA/2J male mouse was utilized as RSA animal model. Pregnant RSA mice were injected different types of lymphocytes through tail vein. Independent sample t-test was used to analyze the data from each group. Results: The proportion of CD4+CD25+ T cells in CD4+T cells was (17.49 ± 0.60)％in CD4+CD25+ regulatory T cells injection group, which was statistical significant higher than that of mononuclear lymphocyte injection group (14.68 ± 0.83)％, sterile PBS group (9.54 ±