Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredox

Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in

Mytilus galloprovincialis

WANG Qing;NING Xuanxuan;PEI Dong;ZHAO Jianmin;YOU Liping;WANG Chunyan;WU Huifeng

【期刊名称】《中国海洋湖沼学报（英文版）》 【年(卷),期】2013(031)003

【摘要】Thioredoxin (Trx) proteins are involved in many biological processes especially the regulation of cellular redox homeostasis.In this study,two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR).The two cDNAs were named MgTrx1 and MgTrx2,respectively.The open reading frames of MgTrx1 and MgTrx2 were 318 and 507 base pairs (bp) and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa,respectively.Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys.In addition,MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria.Quantitative real-time polymerase chain reaction (qPCR) revealed that both MgTrx1 and MgTrx2 were constitutively expressed in all tissues examined.The MgTrxl transcript was most abundant in hemocytes and gills,whereas