Cloning and characterization of a novel deletion mutant of heterogeneous nuclear ribonucle

Cloning and characterization of a novel deletion

mutant of heterogeneous nuclear

ribonucleoprotein M4 from human dendritic cells

作者：黄欣;赵忠良;袁正隆;张明徽;朱学军;陈国友;曹雪涛;

作者机构：Department of Immunology;Second Military Medical University;Shanghai 200433;China;China;;; 来源：中国科学 年：2000 卷：000 期：006

页码：P.648-654 页数：7 中图分类：Q785 正文语种：CHI

关键词：dendritic cells subtractive hybridization gene cloning heterogeneous nuclear ribonucleoprotein deletion mutant. 摘要：To identify differentially expressed genes from antigen-stimulated human dendritic cells (DC), subtractive cloning was adopted and more than ten novel genes differentially expressed were cloned. One is a deletion mutant of heterogeneous nuclear ribonucleoprotein (hnRNP) M4 in which the residues from 159 to 197 of hnRNP M4 have been absent. The deletion mutant was shown to- be co-expressed with hnRNP M4 in cell lines. The mutant was expressed in antigen-stimulated DC but not in normal DC. Northern blot analysis revealed the presence of a major hnRNP M4 deletion mutant mRNA transcript of 2.4 kilobase