Comparative Analysis of the Endosperm Proteins Separated by 2-D Electrophoresis for Two Cu

Comparative Analysis of the Endosperm Proteins Separated by 2-D Electrophoresis for Two Cultivars

of Hybrid Rice (Oryza sativa L.)

Pingfang Yang;Shihua Shen;Tingyun Kuang

【期刊名称】《植物学报（英文版）》 【年(卷),期】2006(048)009

【摘要】Liangyoupeijiu is a two-parental-line, and Shanyou63 is a three-parental-line hybrid rice (Oryza sativa L.).Although both belong to the indica subspecies, they have obvious differences with respect to morphology,physiology and grain quality. Variations in endosperm protein

compositions

were

studied

by

comparing

the

2-D

electrophoresis (2-DE) maps for these two cultivars of hybrid rice. After matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) analysis, a 21-kDa precursor of 19-kDa globulin was identified as the major storage protein for both cultivars. Some isoforms of peroxiredoxin and seed maturation protein were found to only exist in Shanyou63, whereas aldose reductase and starch granule-bound starch synthase were only detected in Liangyoupeijiu. These data might provide a foundation for further comparative studies of these two cultivars of hybrid rice. 【总页数】6页(1028-1033)

【关键词】endosperm;hybrid rice;mass spectrometry;matrix assisted

laser desorption/ionization time of flight;2-D electrophoresis 【作者】Pingfang Yang;Shihua Shen;Tingyun Kuang

【作者单位】Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China;Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China

【正文语种】中文 【中图分类】Q94 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_journal-integrative-plant-biology_thesis/0201255533817.html 【相关文献】

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