地塞米松对高氧肺损伤新生大鼠肺水通道蛋白1表达的影
响
陈国兵;许峰;卢仲毅;匡凤梧;王兴勇
【期刊名称】《中国小儿急救医学》 【年(卷),期】2008(015)005
【摘要】Objective To investigate the effect of dexamethasone on aquporin-1(AQP1) expression in the lung of hyperoxia-exposed newborn rats and to explore the protective mechanism of dexamethasone in lung injury.Methods Thirty-two newhorn Wistar rats were randomly divided into 4 groups:room-air group, hyperoxia-exposure group, room-air group treated with dexamethasone and hyperoxia-exposure group treated with dexamethasone. Lungs were harvested on the third day. The lung AQP1 mRNA expression and the protein location were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. In addition, the wet-to-dry weight ratio(W/D), the protein content of broncho alveolar lavage fluid (BALF), lung permeation index and histological changes in lung were measured respectively. Results On the third day after hyperoxia-exposure, lung tissues appeared congestion, inflammatory cells exudation and edema. The lung W/D, the protein content in BALF and the lung permeation index in hyperoxia group were significantly higher than those in room-air group. In contrast, lung injury degree
地塞米松对高氧肺损伤新生大鼠肺水通道蛋白1表达的影响
