威宁绵羊IGF-1基因外显子多态性分析
刘标;易鸣;张依裕;王振;程朝友;杨德文;程均华;付正仙;王进
【期刊名称】《山地农业生物学报》 【年(卷),期】2017(036)002
【摘要】In order to protect and utilize the fine local variety,DNA pool of Weining sheep was constructed.4 primers were designed to amplify exon and intron sequences of IGF-1 gene in Weining sheep.Products of PCR were purified and bidirectional sequenced to determine the SNPs.Influence of polymorphic sites on the RNA secondary and tertiary structure of IGF-1 gene was analyzed by online bioinformatics software.The result showed that 3 SNPs were detected in IGF-1 gene,namely
Exon2-T87C,a
synonymous
mutation;Exon4-A27T,a
missense mutation which changed the code of Arg into Ser;and Intron1-A4387C,a noncoding mutation in the intron.%为了更好地保护和开发利用威宁绵羊这一优良地方品种,本实验利用威宁绵羊构建DNA池,设计4对引物扩增威宁绵羊IGF-1基因外显子及部分内含子序列.将PCR产物纯化并进行双向测序,通过DNAStar生物软件分析确定多态性位点.利用在线生物信息学软件分析多态位点对IGF-1基因RNA的二级结构、类胰岛素生长因子1的二级和三级结构的影响.结果表明,在扩增的IGF-1基因中筛选到3个SNPs:Intron1-A4387C、Ex-on2-T87C、Exon4-A27T,其中Exon4-A27T为错义突变,导致编码的精氨酸(Arg)变为丝氨酸(Ser);Exon2-T87C多态位点均未改变氨基酸的编码,为同义突变;Intron1-A4387C在内含子区,不参与氨基酸编码.
威宁绵羊IGF-1基因外显子多态性分析
