xTAG(R) GPP多重核酸技术在北京地区感染性腹泻诊断中
的应用及流行病学研究
冯伟明;顾秀丽;隋文君;张明新;鲁炳怀;王玫;黄艳飞;鲁辛辛
【期刊名称】《中华医学杂志》 【年(卷),期】2015(095)006
【摘要】Objective To investigate the application value of xTAG(R) gastrointestinal pathogen panel (xTAG9(R) GPP) multiplex PCR in the early diagnosis of infectious diarrhea,and understand the epidemiology of intestinal diarrhea pathogens.Methods Five hundred and ninety two specimens were collected in outpatient of Tongren Hospital,Capital Medical University,from 1st Oct 2013 to 30th Sep 2014,comparing the xTAG(R) GPP multiplex PCR assay with the traditional methods (culture,rapid
enzyme
immunoassay
chromatography,microscopic
examination,Real-time PCR) and mading the statistical analysis.Results The positive rate of 592 patients with diarrhea specimens was 47.8％ (283/592),the proportion of male and female was 1:1.02,the average age was 31 years.The virus detection rate was 18.1％,Rotavirus A was the most common organism detected(8.8％),concentrated in winter,popular in children.Secondly,Norovirus G Ⅰ/G Ⅱ (8.4％),Adenovirus 40/41 was five cases.The positive rate of bacteria was 35.5％,Enterotoxigenic E.coli (8.4％,50/592) was most frequently detected in summer,common in young
adults.The
other
pathogens
were
Campylobacter
7.7％,Salmonella 7.0％,Clostridium difficile toxinA/B 3.5％,Shigella 3.3％,E.coli O157 3.3％ and Shiga toxin-producing E.coli LT/ST 1.7％.None of Yersinia enterocolitica and Vibrio cholerae was detected.There were ten samples with parasitic (1.7％),five samples were positive for Cryptosporidium,three for Entamoeba histolytica and two for Giardia.All of them did not have obvious distribution followed by season and population.Totally 242 (40.8％) infected specimens with single pathogen were detected.There were 41 (6.9％) co-infections samples,including two pathogens 36 cases (6.1％),three pathogens in 5 cases
(0.8％).Conclusions
xTAG(R)
GPP
multiplex
PCR
is
simple,sensitive,specific and can be used as a quick way to diagnose the infectious diarrhea.Diarrhea pathogen has significant characteristics with the season and crowd.%目的 探讨xTAG(R) GPP多重核酸技术对感染性腹泻早期诊断的应用价值,了解肠道腹泻病原体的流行病学情况.方法 收集2013年10月1日至2014年9月30日于首都医科大学附属北京同仁医院门诊就诊的腹泻患者便标本592份,运用xTAG(R) GPP法进行检测,将其结果与传统方法(培养、快速酶免疫层析法、显微镜检查、实时荧光定量PCR等)比较,并进行统计学分析.结果 xTAG(R) GPP法检测592份便标本的阳性率为47.8％ (283/592),男女比例1:1.02,平均发病年龄31岁.病毒检出率为18.1％,以轮状病毒A型(RotV A)为主(8.8％),主要集中在冬季,儿童多发,其次为诺如病毒G Ⅰ/GⅡ(NorV G Ⅰ/GⅡ)占8.4％,腺病毒40/41(AdeV 40/41)检出5份.细菌阳性率为35.5％,产毒型大肠埃希菌LT/ST型(ETEC)最常见(8.4％,50/592),夏季高
发,青壮年易感,其他病原体依次为弯曲杆菌(Camp)7.7％、沙门菌(Salm) 7.0％、难辨梭状芽胞杆菌毒素A/B型(C.difA/B) 3.5％、志贺菌(Shig) 3.3％、0157型大肠埃希菌(E.coli 0157)3.3％及产志贺毒素大肠埃希菌stx1/stx2型(STEC-LT/ST) 1.7％,未检出小肠结肠炎耶尔森氏菌(Y.ent)与霍乱弧菌(V.cho).检出寄生虫10份(1.7％),包括隐孢子虫(Crypto)5份、痢疾阿米巴(E.his)3份及贾第虫(Gia)2份,均无明显的季节和人群分布.单一病原体感染患者占40.8％(242/592),混合感染为6.9％(41/592),其中双重感染6.1％(36/592),三重感染0.8％ (5/592).结论 xTAG(R) GPP多重核酸方法操作简便、灵敏、特异,可作为感染性腹泻病原学诊断的快速方法.腹泻病原体具有显著的季节和人群优势. 【总页数】5页(435-439)
【关键词】腹泻;核酸扩增技术;病毒;细菌;寄生虫
【作者】冯伟明;顾秀丽;隋文君;张明新;鲁炳怀;王玫;黄艳飞;鲁辛辛
【作者单位】100710 首都医科大学附属北京同仁医院检验科;100710 首都医科大学附属北京同仁医院检验科;100710 首都医科大学附属北京同仁医院检验科;100710 首都医科大学附属北京同仁医院检验科;民航总医院检验科;100710 首都医科大学附属北京同仁医院检验科;100710 首都医科大学附属北京同仁医院检验科;100710 首都医科大学附属北京同仁医院检验科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_national-medical-journal-china_thesis/0201254861387.html
xTAG(R) GPP多重核酸技术在北京地区感染性腹泻诊断中的应用及流行病学研究
