Photoperiod-and thermo-sensitive genic male sterility in rice are caused by a point mutati

Photoperiod-and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

Hai Zhou;Zhenlan Liu;Letian Chen;Yao-Guang Liu;Chuxiong Zhuang;Qinjian Liu;Jing Li;Dagang Jiang;Lingyan Zhou;Ping Wu;Sen Lu;Feng Li;Liya Zhu

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2012(022)004

【摘要】Photoperiod- and thermo-sensitive genic male sterility (PGMS and TGMS) are the core components for hybrid breeding in crops.Hybrid rice based on the two-line system using PGMS and TGMS lines has been successfully developed and applied widely in agriculture.However,the molecular mechanism underlying the control of PGMS and TGMS remains obscure.In this study,we mapped and cloned a major locus,p/tms12-1 (photo- or thermo-sensitive genic male sterility locus on chromosome 12),which confers PGMS in the japonica rice line Nongken 58S (NK58S)and TGMS in the indica rice line Peiai 64S (PA64S,derived from NK58S).A 2.4-kb DNA fragment containing the wild-type allele P/TMS12-1 was able to restore the pollen fertility of NK58S and PA64S plants in genetic complementation.P/TMS12-1 encodes a unique noncoding RNA,which produces a 21-nucleotide small RNA that we named osa-smR5864w.A substitution of C-to-G in p/tms12-1,the only polymorphism relative to P/TMS12-1,is present in