人巨细胞病毒临床低传代病毒株D2的UL140基因B细胞
表位生物信息学特征
苏海浩;王波;郭梦杰;谭琪琪;胡兢晶;伍苑宾;晏翠芳
【期刊名称】《中华妇幼临床医学杂志(电子版)》 【年(卷),期】2017(013)006
【摘要】Objective To investigate the bioinformatics characteristics of B cell epitopes of UL140 gene of human cytomegalovirus(HCMV) clinical low-passage virus strain D2 (to call for short as HCMV D2) isolated from infected neonates in Guangdong Provincial Maternal and Child Health Institute .Methods The HCMV D2 isolated from 10 fresh urine samples of 10 neonates with HCMV positive from Neonatology department in Guangdong Provincial Maternal and Child Health Institute were selected as research materials . Applied bioinformatics methods , including analyzed the gene transmembrane structure by Hidden Markov Model (TMHMM );predicted gene hydrophilicity , surface accessibility , polarity , flexibility and antigenicity of UL140 gene through ExPASy online sequence analysis platform ;the Garnier-Osguthorpe-Robson (GOR ) , hierarchical neutral network (HNN) and sawmill owners and plywood manufacturers association (SOPMA) were used to predict the secondary structure of UL140 gene .Aggregate analyzed the results above ,and calculated the antigen index (AI ) of the amino acid residues translated by UL140 gene , then summarized bioinformatics of B cell epitopes of