腺病毒E1A蛋白对核因子-κB活化的影响及N-乙酰半胱氨
酸的干预作用
陈娟;李冰;冉丕鑫
【期刊名称】《中华结核和呼吸杂志》 【年(卷),期】2010(033)001
【摘要】Objective The relationship between latent adenvorius infection and airway inflammation had not been well documented.The aim of this study was to illustrate the roles of adenovirus E1 A protein on the transactivation of NF-κ inflammatory stimuli and the effect of N-Acetylcysteine (NAC)upon the transactivation of NF-κB and AP-1 in cells stably expressing E1 A protein.Methods Rat alveolar epithelial cells stably expressing adenoviral E1 A or control plasmid were developed.For isolation of nuclear extracts,5×10~5 cells were plated and grown overnight in 60 mm dishes.Experiments were repeated 3 times.The cell model of stably expressing adenoviral E1 A was stimulated by LPS or TNF-aκ and treated with NAC,a precursor for cysteine.The NF-κB and AP-1 transcriptional activity were measured by LUC report system.The expression of NF-κB and AP-1 were measured by Western blot.Differences between groups were assessed for significance by Student't test,and multiple comparisons were made by one-way ANOVA.Results The luciferage activity drived by NF-κB element wag(9 698±98)RLU
in
untreated
E1
A-positive
clones
and(101
腺病毒E1A蛋白对核因子-κB活化的影响及N-乙酰半胱氨酸的干预作用
