芡实提取物对SH-SY5Y神经细胞损伤的保护作用及体外抗
氧化活性研究
沈蓓;袁冬平;伍城颖;贺潇潇;王新胜;吴启南
【期刊名称】《南京中医药大学学报》 【年(卷),期】2013(029)001
【摘要】OBJECTIVE To investigate the protective effects of euryale seed 80% ethanol, ethyl acetate and n-butyl alcohol extracts on the H2 O2-induced damaged SH-SY5Y neurocytes and their invitro antioxidant activities. METHODS The H2O2-induced damaged SH-SY5Y neurocyte models were cultured outside of the body. Cell Counting Kit-8 (CCK-8) was used to determine the effects of the above euryale seed extracts with different concentrations on the activities of the H2O2-induced damaged SH-SY5Y neurocytes, and then their inhibition rates were calculated. The in-vitro antioxidant experimental method was applied to detecting the capabilities of the three extracts in removing DPPH, ABTS radicals and deoxidating three price iron i-ons. RESULTS Within the crude drug concentration range of 10~30mg/mL, euryale seed extracts in 80% ethanol, ethyl acetate and n-butyl alcohol showed obvious functions of inhibiting the oxidant damage action of H2O2 on the SH-SY5Y neurocytes (P<0.05, P<0.01). In addition, the above euryale seed extracts could markedly clear away DPPH, ABTS radicals and deoxidate three price iron ions, and among which, the n-butyl alcohol extract had
芡实提取物对SH-SY5Y神经细胞损伤的保护作用及体外抗氧化活性研究
