Evidence for a conserved microbiota across the different developmental stages of Plodia in

Evidence for a conserved microbiota across the

different developmental stages of Plodia

interpunctella

Valeria Mereghetti;Bessem Chouaia;Lidia Limonta;Daria Patrizia Locatelli;Matteo Montagna

【期刊名称】《中国昆虫科学：英文版》 【年(卷),期】2024(026)003

【摘要】Diversity and composition of lepidopteran microbiotas are poorly investigated,especially across the different developmental stages.To improve this knowledge,we characterize the microbiota among different developmental stages of the Indian meal moth,Plodia interpunctella,which is considered one of the major pest of commodities worldwide.Using culture-independent approach based on Illumina 16S rRNA gene sequencing we characterized the microbiota of four developmental stages:eggs,first-,and last-instar larvae,and adult.A total of 1022 bacterial OTUs were obtained,showing a quite diversified microbiota associated to all the analyzed stages.The microbiotas associated with P.interpunctella resulted almost constant throughout the developmental stages,with approximately 77％ of bacterial OTUs belonging to the phylum of Proteobacteria.The dominant bacterial genus is represented by Burkholderia (～64％),followed by Propionibacterium,Delftia,Pseudomonas,and Stenotrophomonas.A core