Overexpressions of Lambda Phage Lysis Genes and Biosynthetic Genes of Poly-β-hydroxybutyr

Overexpressions of Lambda Phage Lysis Genes and Biosynthetic Genes of Poly-β-hydroxybutyrate in

Recombinant E.coli

佚名

【期刊名称】《清华大学学报（英文版）》 【年(卷),期】1999(004)002

【摘要】A plasmid (pTU9) containing the lambda (λ) phage lysis genes S(-)RRz and the biosynthetic genes phbCAB of poly-β-hydroxybutyrate (PHB) was constructed and transformed into E.coli JM109. Cultured in Luria-Bertani (LB) medium with 20 g/L glucose, E.coli JM109 (pTU9) could accumulate PHB in cells up to 40% (g PHB per g dry cells). A chelating agent EDTA was applied to induce a complete cell lysis and PHB granules were released. This method has a potential application in PHB separation.

【总页数】5页(1472-1476)

【关键词】poly-β-hydroxybutyrate (PHB);cell disruption;lambda (λ) phage;lysis gene 【作者】佚名 【作者单位】无 【正文语种】中文 【中图分类】O6 【相关文献】