金针菇生长发育期间相关胞外酶的活性变化研究
冯伟林;蔡为明;金群力;范丽军;沈颖越;宋婷婷;田芳芳
【期刊名称】《浙江农业学报》 【年(卷),期】2012(024)003
【摘要】Changes of five extracellular enzymes activity(carboxymethyl cellulose, hemicellulase, laccase, neutral proteinase and acid phosphatase) of Flammulina velutipes were investigated during its growth and development. The results showed thai the activity curves of extracellular enzyme during the growth and development showed markedly periodic style. Carboxymethyl cellulose activity was relatively low during the my cell al vegetative growth phase but it reached 11.43 U after primordium formation. Hemicellulase activity was the highest of 8. 13 U during the harvest phase. Laccase activity was relatively high during mycelial growth phase and decreased to 0. 15 U after primordium formation. Neutral proteinase activity remained constant during mycelial vegetative growth phase, decreased to relatively low levels before primordium formation, and increased again during the fruit body growth phase. Acid phosphatase activity decreased with mycelium culture, but increased sharply after development formation.%以白色金针菇菌株为材料,研究其生长发育过程中羧甲基纤维素酶、半纤维素酶、漆酶、酸性磷酸酶和中性蛋白酶活性的变化规律.结果表明,整个生长发育期间胞外酶活性变化具有明显的阶段性.羧甲基纤维素酶菌丝营养生长阶段活性较低,现
金针菇生长发育期间相关胞外酶的活性变化研究
