骨膜复合骨髓间充质干细胞和软骨细胞体内移植修复软骨
缺损
刘吉斌;侯连东;于强;白毅;王彦东
【期刊名称】《中国组织工程研究》 【年(卷),期】2015(000)003
【摘要】背景：以往主要采用磨削灌洗、钻孔、微骨折技术等对软骨缺损组织进行修复，但多数技术被认为具有一定的局限性，只能减轻患者疼痛或仅短期有效。近年来越来越多的研究表明，软骨组织工程修复技术对于软骨再生及修复具有重要意义。
目的：观察自体骨膜复合骨髓间充质干细胞和软骨细胞修复兔膝关节髁间窝软骨缺损的效果。
方法：取新西兰大白兔28只，随机均分为两组，建立双侧膝关节髁间窝关节软骨缺损模型，实验组左侧植入自体骨膜与骨髓间充质干细胞-软骨细胞复合物，右侧不植入任何材料作为空白对照；对照组左侧植入自体骨膜，右侧不植入任何材料作为空白对照。术后第8，12周，取膝关节软骨缺损部位新生组织，行大体、组织学观察及Wakitani评分。
结果与结论：术后12周，实验组修复组织表面基本光滑，与周围软骨色泽极其相近，出现大量软骨细胞及软骨陷窝，形成软骨基质，Ⅱ型胶原免疫组织化学染色呈阳性，甲苯胺蓝染色较深；对照组修复组织仍为白色，新生修复组织局部凹陷，表面欠光滑，质地较硬，仅有极少量软骨细胞，甲苯胺蓝染色较浅，Ⅱ型胶原免疫组织化学染色呈阴性，无软骨基质形成；空白对照组软骨缺损塌陷，表面不规则，修复组织为纤维组织，甲苯胺蓝染色较浅。表明自体骨膜复合骨髓间充质干细胞与软骨细胞移植能够较好修复关节软骨缺损。oCKGROUND:The main strategies for cartilage repair
include abrasion and lavaging, driling, microfracture technology. But most of these technologies are considered certain limitations that can only aleviate pain and have short-term effects. Cartilage tissue engineering technology has great significance and broad application prospects for cartilage regeneration and repair.
OBJECTIVE:To investigate the effect of periosteum combined bone marrow mesenchymal stem cels and chondrocytes on the repair of articular cartilage defects in the intercondylar fossa of the rabbit knee joints.
METHODS:Bone marrow mesenchymal stem cels and chondrocytes were isolated and purified from rabbits. Twenty-eight New Zealand white rabbits were enroled to create articular defects in the intercondylar fossa with peiosteal coverage in both knee joints, and then these rabbits were randomly divided into two groups: experimental and control groups. The defects in the left side were treated with transplantation of periosteum combined with bone marrow mesenchymal stem cels and chondrocytes or autologous periosteum in the experimental and control groups, respectively, while the defects in the right side received no treatment as blank controls. These rabbits were kiled at 8 and 12 weeks after surgery in each group and newborn tissue samples at defect site were taken for gross observation, histological observation and Wakitani scoring.
RESULTS AND CONCLUSION:At 12 weeks after surgery, in the experimental group, the
repaired tissues were substantialy smooth with a very similar color to the surrounding cartilage tissues, a large number of chondrocytes and cartilage lacunae were visible to form the cartilage matrix, type II colagen immunohistochemical staining was positive, and the tissue was stained darker with toluidine blue; in the control group, the repair tissues were stil white, the newborn tissues presented with local depression, less smooth surface, and hard texture, there was only a very smal amount of chondrocytes, toluidine blue staining was shalow, type II colagen immunohistochemical staining was negative, and no cartilage matrix formed; in the blank control group, the cartilage defects colapsed with irregular surface, the repaired tissues were fibrous tissues and stained lighter with toluidine blue. These findings indicate that autologous periosteum combined with bone marrow mesenchymal stem cels and chondrocytes can effectively repair articular cartilage defects.
【总页数】6页(364-369)
【关键词】生物材料;软骨生物材料;骨膜;软骨缺损;骨髓间充质干细胞;软骨细胞 【作者】刘吉斌;侯连东;于强;白毅;王彦东
【作者单位】大连大学附属中山医院骨科，辽宁省大连市 116001;大连大学附属中山医院骨科，辽宁省大连市 116001;大连大学附属中山医院骨科，辽宁省大连市 116001;大连大学附属中山医院骨科，辽宁省大连市 116001;大连大学附属中山医院骨科，辽宁省大连市 116001
骨膜复合骨髓间充质干细胞和软骨细胞体内移植修复软骨缺损
