TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene

TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene

cluster in Streptomyces aureofaciens

Naxin Wu;He Huang;Taoling Min;Haifeng Hu

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2017(049)012

【摘要】6-Demethylchlortetracycline (6-DCT),a tetracycline antibiotic produced by Streptomyces aureofaciens,is a crucial precursor employed for the semi-synthesis of tigecycline,minocycline,and amadacyclin (PTK 0796).In this study,the 6-DCT biosynthetic gene cluster (BGC) was cloned

from

genomic

DNA

of

a

high

6-DCT-producing

strain,S.aureofaciens DM-1,using the transformation-associated

recombination method.An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S.aureofaciens DM-1.Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34％. 【总页数】6页(1129-1134) 【关键词】

【作者】Naxin Wu;He Huang;Taoling Min;Haifeng Hu

【作者单位】School of Pharmacy, Department of Pharmacology, Fudan University, Shanghai 201203, China;Shanghai Institute of Pharmaceutical Industry, Department of Biopharmceutical, Shanghai 201203, China;Key