relationship between Government and business. The \law enforcement departments and individual window units power for personal gains and bribes, thick friends, relations, human cases, money cases in which seriously infringe on the legitimate rights and interests of enterprises and people. Especially some law enforcement and inspection for profit purposes, deliberately looking for corporate loopholes, found directly under the ticket does not give business improvement opportunities. We recruit a company doesn't easily, cultivating a business more difficult, never for personal gain, systematic harassment, card, last checked to check to get the enterprise collapsed. To guarantee the legitimate rights and interests. Always proceed from the overall situation of reform and development of services, fully consider the characteristics of production and management in non-public enterprises and social benefits, an accurate grasp of legal limits, protect the enthusiasm of entrepreneurs and practitioners in innovation and entrepreneurship, enhancing expectations and confidence. To promote strict enforcement. Strengthening law enforcement procedures, improve law enforcement performance evaluation, strengthening the supervision of law enforcement, exploration and practice of risk control mechanism of law enforcement to further standardize the social security, urban management, road traffic administrative law enforcement, effectively free, extensive law-enforcement and lax enforcement of law enforcement and other issues. To uphold justice. Justice is the last line of Defense to safeguard fairness and justice, to unequivocally support the courts and procuratorates independently exercise level ... Judicial activities were rampant, must not be allowed to run, favors, money, must not be allowed to knowingly violate the miscarriage, violations of the legitimate rights and interests of the masses must not be allowed to abuse of power, to make forest mass can be felt in every judicial cases in equity and justice. Four to deepen grass-roots governance according to lawchain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \visits, like to spend money and buy stop and stable, but disregard the law administration, in accordance with the law, the rulechain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \complex issues, often speak of \专题4《酶的研究与应用》单元测试卷（时间：60分钟 满分：100分）
一、选择题（每题只有一个正确答案。1~10题，每题2分；11~18题，每题2.5分；共40分）
1.从几千年前到现在，酶制剂就安全地在被使用在工业、美容和口腔护理产品、纺织品、食品（如酸奶酪）以及芝士、啤酒和白酒等的发酵生产过程中。下列各项中，不属于酶制剂的是（ ）
A.蛋白酶 B.脂肪酶 C.淀粉酶 D.麦芽糖酶
1.D 解析：目前常用的酶制剂有四大类：蛋白酶、脂肪酶、淀粉酶和纤维素酶。其中，应用最广泛、效果最明显的是碱性蛋白酶和碱性脂肪酶。碱性蛋白酶能将血渍、奶渍等含有的大分子蛋白质水解成可溶性的氨基酸或小分子的肽，使污渍从衣物上脱落。脂肪酶、淀粉酶和纤维素酶也能分别将大分子的脂肪、淀粉和纤维素等水解成小分子物质，使洗衣粉具有更强的去污能力。
2.洗衣粉中的酶是一种生物制剂,它能将不溶于水的某些污渍、污垢分解,使之变成可溶物而被去除从而使衣物变得干净整洁。下列质地的衣物不能用加酶洗衣粉洗涤的是（ ）
A.化纤类衣物 B.棉麻类衣物 C.尼龙类衣物 D.毛类衣物
2.D 解析:加酶洗衣粉含有的蛋白酶较多，蛋白酶可将蛋白质分解，所以蛋白质纤维织物不能用加酶洗衣粉洗涤。
3.植物细胞壁的主要成分是纤维素和果胶，在植物细胞工程中常常用纤维素酶和果胶酶除去细胞壁。下列有关果胶酶的说法中，其中正确的一项是（ ）A.果胶酶可以分解细胞壁的主要成分——纤维素
B.果胶酶是由半乳糖醛酸聚合而成的一种高分子化合物C.果胶酶不特指某种酶，而是分解果胶的一类酶的总称D.果胶酶的化学本质是蛋白质或RNA
3.C 解析:果胶酶并不特指某一种酶，而是分解果胶的一类酶的总称，包括多聚半乳糖醛酸酶、果胶分解酶和果胶酯酶等。果胶酶的化学本质是蛋白质，其基本单位是氨基酸。果胶酶作为酶的一种，具有一般酶的特性，即专一性、高效性、受温度与酸碱度的影响。因此它只能将果胶分解成可溶性的半乳糖醛酸，而不能将纤维素分解为葡萄糖。
4. 固定化酶和固定化细胞是利用物理或化学方法将酶或细胞固定在一定空间的技术，其中适合细胞固定的方法是( )
A.包埋法 B.物理吸附法 C.化学结合法 D.高温冷却法
4.A 解析：主要考查有关固定化酶和固定化细胞的识记内容。固定化酶有物理吸附法和化学结合法，而固定化细胞最适合的方法是包埋法。5.下列有关制备固定化酵母细胞制备步骤中，不正确是（ ）A.在向溶化好的海藻酸钠溶液中加入的必须是已活化的酵母细胞B.溶化好的海藻酸钠溶液必须冷却至室温，才能加入细胞C.溶解海藻酸钠，最好采用小火，或者持续加热的方法
D.在固定化酵母细胞的过程中，要以恒定的速度缓慢地将注射器中的溶液滴加到配制好的CaCl2溶液中
5.A 解析：由于酵母菌是一种真核微生物，温度较高时会死亡。
6. 酶制剂对使用条件是有某些具体要求的，如使用温度、PH值等等。下列有关探究温度和pH对酶活性的影响实验中，不正确的说法是（ ）
Grass-roots governance according to law is an important foundation for promoting the construction of rule of law, but also the most basic development environment. Despite my good social order in General, but there are law-abiding consciousness, social order is not standard, regardless of faith, Twining visit visit and other outstanding problems. We want to actively promote the field of multi-level governance according to law, promote the continuous improvement of the social environment. To promote universal compliance. Actively foster the rule of law culture, carry out law publicity and education on honesty and trustworthiness, guide the masses and consciously abide by the law, failing to find method, problem-solving method, method of gradually changing the world, he is not looking for but someone \against violence crime, mafia and serious criminal offences, to protect the legitimate rights and interests of citizens, legal persons, carry out criminal policy of temper justice with mercy, the maximum stimulating social vitality, and earnestly safeguard social harmony and stability. To resolve social conflicts by law. To further smooth channels of reporting social conditions and public opinion, improving regulation, arbitration, as well as dispute resolution mechanisms, implement fully the petitioners ' cases \should want to do work and able to do good, enthusiasm is paramount. Treat party cadres, it is necessary to Strict management, and warm care, enable the broad masses of cadres work hard work, this is two parallel principles. In strict accordance with the standards of good cadres selection and appointment, the real officers, dare officers, those who want to play, good as excellent cadres at all levels of leadership in the past. At present, some leading cadres work initiative is not high, beyond political, idle, lazy governance governance, not as, slow, messy and a phenomenon still exist to varying degrees, affected and restricted economic and social development. We must mobilize the enthusiasm of cadres as an urgent task, adhere to combination of incentives and constraints, and adhere to the strict management and warm care, practical solution \dare to play, good at shows as new. To identify the problem, accurate pulseof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \investors, and must not break faith with the people, promises and the promise to the society. To build a newof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \by the law makes the right to statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \companies and investors, and must not break faith with the people, promises and the promise to the society. To build a newrelationship between Government and business. The \law enforcement departments and individual window units power for personal gains and bribes, thick friends, relations, human cases, money cases in which seriously infringe on the legitimate rights and interests of enterprises and people. Especially some law enforcement and inspection for profit purposes, deliberately looking for corporate loopholes, found directly under the ticket does not give business improvement opportunities. We recruit a company doesn't easily, cultivating a business more difficult, never for personal gain, systematic harassment, card, last checked to check to get the enterprise collapsed. To guarantee the legitimate rights and interests. Always proceed from the overall situation of reform and development of services, fully consider the characteristics of production and management in non-public enterprises and social benefits, an accurate grasp of legal limits, protect the enthusiasm of entrepreneurs and practitioners in innovation and entrepreneurship, enhancing expectations and confidence. To promote strict enforcement. Strengthening law enforcement procedures, improve law enforcement performance evaluation, strengthening the supervision of law enforcement, exploration and practice of risk control mechanism of law enforcement to further standardize the social security, urban management, road traffic administrative law enforcement, effectively free, extensive law-enforcement and lax enforcement of law enforcement and other issues. To uphold justice. Justice is the last line of Defense to safeguard fairness and justice, to unequivocally support the courts and procuratorates independently exercise level ... Judicial activities were rampant, must not be allowed to run, favors, money, must not be allowed to knowingly violate the miscarriage, violations of the legitimate rights and interests of the masses must not be allowed to abuse of power, to make forest mass can be felt in every judicial cases in equity and justice. Four to deepen grass-roots governance according to lawchain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \visits, like to spend money and buy stop and stable, but disregard the law administration, in accordance with the law, the rulechain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \complex issues, often speak of \A.要一系列的温度梯度和一系列的pH梯度B.注意应用对照原则 C.注意应用单一变量原则
D.研究温度或pH时，都是温度和pH为变量，其他为常量
6．D 解析:研究温度或pH时，控制的自变量分别是温度、pH，酶的活性为因变量，其他的为无关变量或额外变量，应予以严格控制。
7.某同学探究温度对加酶洗衣粉洗涤效果的影响，选择了10℃、20℃、30℃、40℃、50℃、60℃和70℃的温度梯度，在其他条件都一致的情况下，发现30℃时洗涤效果最好，由此可以得出（ ）A.30℃为该酶的最适温度 C. 30℃时，酶活性最高
B.30℃不是该酶的最适温度D.不能确定该酶的最适温度
7. D 解析：该同学的实验符合实验设计原则，对于最适温度要明确的是同一加酶洗衣粉，也与洗涤时间长短有关。若温度梯度差太大，因而只能说在该条件下30℃时洗涤效果最好。至于最适温度，应围绕30℃再设置较小梯度差进一步测定。
8.固定化酶技术是近几年新兴发展起来的一项酶应用技术，它很好的解决了酶的重新利用的能力，大大提高了酶的利用率，从而避免了酶的浪费问题。下列关于固定化酶技术的说法正确的是( )
A.固定化酶技术就是固定反应物,将酶依附着载体围绕反应旋转的技术B.固定化酶的优势在于能催化一系列的酶促反应C.固定化酶中的酶无法重复利用
D.固定化酶是将酶固定在一定空间内的技术
8.D 解析：固定化酶是利用物理或化学方法将酶固定在一定空间内的技术，其优点是酶被固定在一定装置内可重复利用，不足是无法同时解决一系列酶促反应。
9.日常生活中我们用的加酶洗衣粉种类很多，它不仅能够除去衣物的奶渍，而且还能够去除一些血渍，这主要是因为它含有（ ）A.脂肪酶 B.蛋白酶 C.淀粉酶 D.氧化酶
9．B 解析：奶渍和血渍的“痕迹”成分主要是蛋白质，所以应考虑是蛋白酶。10.在用果胶酶处理果泥时，为了使果胶酶能够充分地催化反应，应采取的措施是（ ）
A.加大苹果泥用量 B.加大果胶酶用量C.进一步提高温度 D.用玻璃棒不时地搅拌反应混合物
10．D 解析：搅拌可以使酶与底物充分接触而提高反应效率，使反应更充分。11.（2008广东生物）关于蛋白酶的叙述中，不正确的是（ ）A.蛋白酶是蛋白质
B.蛋白酶可以作为药品治疗某些疾病C.蛋白酶可以水解所有的肽键
D.利用酶工程可以提高蛋白酶的稳定性
11.C 解析：主要考查蛋白酶的化学本质、作用部位以及应用。蛋白酶是蛋白
Grass-roots governance according to law is an important foundation for promoting the construction of rule of law, but also the most basic development environment. Despite my good social order in General, but there are law-abiding consciousness, social order is not standard, regardless of faith, Twining visit visit and other outstanding problems. We want to actively promote the field of multi-level governance according to law, promote the continuous improvement of the social environment. To promote universal compliance. Actively foster the rule of law culture, carry out law publicity and education on honesty and trustworthiness, guide the masses and consciously abide by the law, failing to find method, problem-solving method, method of gradually changing the world, he is not looking for but someone \against violence crime, mafia and serious criminal offences, to protect the legitimate rights and interests of citizens, legal persons, carry out criminal policy of temper justice with mercy, the maximum stimulating social vitality, and earnestly safeguard social harmony and stability. To resolve social conflicts by law. To further smooth channels of reporting social conditions and public opinion, improving regulation, arbitration, as well as dispute resolution mechanisms, implement fully the petitioners ' cases \should want to do work and able to do good, enthusiasm is paramount. Treat party cadres, it is necessary to Strict management, and warm care, enable the broad masses of cadres work hard work, this is two parallel principles. In strict accordance with the standards of good cadres selection and appointment, the real officers, dare officers, those who want to play, good as excellent cadres at all levels of leadership in the past. At present, some leading cadres work initiative is not high, beyond political, idle, lazy governance governance, not as, slow, messy and a phenomenon still exist to varying degrees, affected and restricted economic and social development. We must mobilize the enthusiasm of cadres as an urgent task, adhere to combination of incentives and constraints, and adhere to the strict management and warm care, practical solution \dare to play, good at shows as new. To identify the problem, accurate pulseof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \investors, and must not break faith with the people, promises and the promise to the society. To build a newof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \by the law makes the right to statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \companies and investors, and must not break faith with the people, promises and the promise to the society. To build a newrelationship between Government and business. The \law enforcement departments and individual window units power for personal gains and bribes, thick friends, relations, human cases, money cases in which seriously infringe on the legitimate rights and interests of enterprises and people. Especially some law enforcement and inspection for profit purposes, deliberately looking for corporate loopholes, found directly under the ticket does not give business improvement opportunities. We recruit a company doesn't easily, cultivating a business more difficult, never for personal gain, systematic harassment, card, last checked to check to get the enterprise collapsed. To guarantee the legitimate rights and interests. Always proceed from the overall situation of reform and development of services, fully consider the characteristics of production and management in non-public enterprises and social benefits, an accurate grasp of legal limits, protect the enthusiasm of entrepreneurs and practitioners in innovation and entrepreneurship, enhancing expectations and confidence. To promote strict enforcement. Strengthening law enforcement procedures, improve law enforcement performance evaluation, strengthening the supervision of law enforcement, exploration and practice of risk control mechanism of law enforcement to further standardize the social security, urban management, road traffic administrative law enforcement, effectively free, extensive law-enforcement and lax enforcement of law enforcement and other issues. To uphold justice. Justice is the last line of Defense to safeguard fairness and justice, to unequivocally support the courts and procuratorates independently exercise level ... Judicial activities were rampant, must not be allowed to run, favors, money, must not be allowed to knowingly violate the miscarriage, violations of the legitimate rights and interests of the masses must not be allowed to abuse of power, to make forest mass can be felt in every judicial cases in equity and justice. Four to deepen grass-roots governance according to lawchain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \visits, like to spend money and buy stop and stable, but disregard the law administration, in accordance with the law, the rulechain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \complex issues, often speak of \质，只有核酸类酶是一类具有生物催化功能的核糖核酸（RNA） 分子；通过酶分子修饰，可以提高酶活力，增加酶的稳定性，消除或降低酶的抗原性，所以利用酶工程可以提高蛋白酶的稳定性；蛋白酶可以作为药品治疗某些消化道疾病；蛋白酶能水解某些氨基酸形成多肽，但不能水解所有的肽键。例如胰蛋白酶仅水解由赖氨酸、精氨酸的羧基与其他氨基酸的氨基形成的肽键，生成多肽。
12. (2009山东实验中学一诊)酶制剂已经广泛地应用于工业、医药等方面，在生产中使用酶制剂时，需要进行酶活性的测定，而适宜的温度是酶活性的保证。下图四条曲线分别表示在四种温度下反应物消耗量随时间的变化。则该酶作用的适宜温度应处于哪一种温度附近（ ）
A．T1 B．T2 C．T3 D．T4 12．A
13.下列说法中，哪项不是在应用酶的过程中常遇见的一些实际问题?( )A.酶与反应物混合，产品难以提纯B.酶在生物化学反应中往往难以回收C.酶遇强酸、强碱、高温等条件易失活D.酶作为催化剂，绝大多数酶是蛋白质
13.D解析：酶的作用条件比较温和，易受温度、酸碱度的影响；酶在生物化学反应中与反应物、产物混合在一起，很难回收，影响产品质量。这些都是酶应用过程中常遇见的一些问题
Grass-roots governance according to law is an important foundation for promoting the construction of rule of law, but also the most basic development environment. Despite my good social order in General, but there are law-abiding consciousness, social order is not standard, regardless of faith, Twining visit visit and other outstanding problems. We want to actively promote the field of multi-level governance according to law, promote the continuous improvement of the social environment. To promote universal compliance. Actively foster the rule of law culture, carry out law publicity and education on honesty and trustworthiness, guide the masses and consciously abide by the law, failing to find method, problem-solving method, method of gradually changing the world, he is not looking for but someone \against violence crime, mafia and serious criminal offences, to protect the legitimate rights and interests of citizens, legal persons, carry out criminal policy of temper justice with mercy, the maximum stimulating social vitality, and earnestly safeguard social harmony and stability. To resolve social conflicts by law. To further smooth channels of reporting social conditions and public opinion, improving regulation, arbitration, as well as dispute resolution mechanisms, implement fully the petitioners ' cases \should want to do work and able to do good, enthusiasm is paramount. Treat party cadres, it is necessary to Strict management, and warm care, enable the broad masses of cadres work hard work, this is two parallel principles. In strict accordance with the standards of good cadres selection and appointment, the real officers, dare officers, those who want to play, good as excellent cadres at all levels of leadership in the past. At present, some leading cadres work initiative is not high, beyond political, idle, lazy governance governance, not as, slow, messy and a phenomenon still exist to varying degrees, affected and restricted economic and social development. We must mobilize the enthusiasm of cadres as an urgent task, adhere to combination of incentives and constraints, and adhere to the strict management and warm care, practical solution \dare to play, good at shows as new. To identify the problem, accurate pulseof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \investors, and must not break faith with the people, promises and the promise to the society. To build a newof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \by the law makes the right to statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \companies and investors, and must not break faith with the people, promises and the promise to the society. To build a newrelationship between Government and business. The \law enforcement departments and individual window units power for personal gains and bribes, thick friends, relations, human cases, money cases in which seriously infringe on the legitimate rights and interests of enterprises and people. Especially some law enforcement and inspection for profit purposes, deliberately looking for corporate loopholes, found directly under the ticket does not give business improvement opportunities. We recruit a company doesn't easily, cultivating a business more difficult, never for personal gain, systematic harassment, card, last checked to check to get the enterprise collapsed. To guarantee the legitimate rights and interests. Always proceed from the overall situation of reform and development of services, fully consider the characteristics of production and management in non-public enterprises and social benefits, an accurate grasp of legal limits, protect the enthusiasm of entrepreneurs and practitioners in innovation and entrepreneurship, enhancing expectations and confidence. To promote strict enforcement. Strengthening law enforcement procedures, improve law enforcement performance evaluation, strengthening the supervision of law enforcement, exploration and practice of risk control mechanism of law enforcement to further standardize the social security, urban management, road traffic administrative law enforcement, effectively free, extensive law-enforcement and lax enforcement of law enforcement and other issues. To uphold justice. Justice is the last line of Defense to safeguard fairness and justice, to unequivocally support the courts and procuratorates independently exercise level ... Judicial activities were rampant, must not be allowed to run, favors, money, must not be allowed to knowingly violate the miscarriage, violations of the legitimate rights and interests of the masses must not be allowed to abuse of power, to make forest mass can be felt in every judicial cases in equity and justice. Four to deepen grass-roots governance according to lawchain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \visits, like to spend money and buy stop and stable, but disregard the law administration, in accordance with the law, the rulechain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \complex issues, often speak of \14.关于固定化酶中的酶说法,正确的是( )A.酶的种类多样，可催化一系列的酶促反应B.酶被固定在不溶于水的载体上，可反复利用
C.酶作为催化剂，反应前后结构不改变，所以固定化酶可永远利用下去D.固定化酶由于被固定在载体上，所以丧失了酶的高效性和专一性特点
14.B 解析:固定化酶是利用物理或化学方法将酶固定在一定空间内的技术，其优点是酶被固定在一定装置内可重复利用，不足是无法同时解决一系列酶促反应。
15.下表是某种生物活性洗衣粉的成分及用法说明，下列对资料表明的信息理解不正确的是（ ）
A.这种洗衣粉较易清除衣服上的蛋清污渍B.该蛋白酶在60℃以上将会失去活性C.这种洗衣粉可除去衣料上的各种污渍D.这种洗衣粉不能用于洗涤真丝织物
15.C 解析：酶具有专一性。因为成分中有蛋白酶，只能洗涤一些含有蛋白质的奶渍等污渍，但不能除去衣料上的其它污渍。16. 下列验证某种加酶洗衣粉需要的最适温度的实验中，正确的一项是（ ）
A．取一系列不同温度、其他条件相同的水，加入相同的污物及等量加酶洗衣粉，看哪一个温度中酶的效果最好
B．在不同温度的水中，加入不等量洗衣粉，看哪种效果好
C．将加酶洗衣粉加入30℃水中发现不如加到45℃水中洗涤效果好，说明45℃为最适温度
D．将加酶洗衣粉与普通洗衣粉分别加入37℃的水中洗涤同样的污物，发现加酶洗衣粉效果好，说明加酶洗衣粉的最适温度为37℃
16．A 解析：本实验的自变量为温度，在对照实验中，除了要观察的变量外，其它变量都应保持相同，B选项错误；C选项可以说明加酶洗衣粉在45℃时比30℃时洗涤效果好，但不能说明45℃为最适温度，C选项错误；D选项只能比较加酶洗衣粉与普通洗衣粉的洗涤效果，不能得出加酶洗衣粉的最适温度，D选项错误。
17.如下图所示，某洗衣粉中有一生物活性物质。为验证其性质，进行了如下实验。下列的有关叙述中，不合理的是（ ）
Grass-roots governance according to law is an important foundation for promoting the construction of rule of law, but also the most basic development environment. Despite my good social order in General, but there are law-abiding consciousness, social order is not standard, regardless of faith, Twining visit visit and other outstanding problems. We want to actively promote the field of multi-level governance according to law, promote the continuous improvement of the social environment. To promote universal compliance. Actively foster the rule of law culture, carry out law publicity and education on honesty and trustworthiness, guide the masses and consciously abide by the law, failing to find method, problem-solving method, method of gradually changing the world, he is not looking for but someone \against violence crime, mafia and serious criminal offences, to protect the legitimate rights and interests of citizens, legal persons, carry out criminal policy of temper justice with mercy, the maximum stimulating social vitality, and earnestly safeguard social harmony and stability. To resolve social conflicts by law. To further smooth channels of reporting social conditions and public opinion, improving regulation, arbitration, as well as dispute resolution mechanisms, implement fully the petitioners ' cases \should want to do work and able to do good, enthusiasm is paramount. Treat party cadres, it is necessary to Strict management, and warm care, enable the broad masses of cadres work hard work, this is two parallel principles. In strict accordance with the standards of good cadres selection and appointment, the real officers, dare officers, those who want to play, good as excellent cadres at all levels of leadership in the past. At present, some leading cadres work initiative is not high, beyond political, idle, lazy governance governance, not as, slow, messy and a phenomenon still exist to varying degrees, affected and restricted economic and social development. We must mobilize the enthusiasm of cadres as an urgent task, adhere to combination of incentives and constraints, and adhere to the strict management and warm care, practical solution \dare to play, good at shows as new. To identify the problem, accurate pulseof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \investors, and must not break faith with the people, promises and the promise to the society. To build a newof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \by the law makes the right to statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \companies and investors, and must not break faith with the people, promises and the promise to the society. To build a newrelationship between Government and business. The \law enforcement departments and individual window units power for personal gains and bribes, thick friends, relations, human cases, money cases in which seriously infringe on the legitimate rights and interests of enterprises and people. Especially some law enforcement and inspection for profit purposes, deliberately looking for corporate loopholes, found directly under the ticket does not give business improvement opportunities. We recruit a company doesn't easily, cultivating a business more difficult, never for personal gain, systematic harassment, card, last checked to check to get the enterprise collapsed. To guarantee the legitimate rights and interests. Always proceed from the overall situation of reform and development of services, fully consider the characteristics of production and management in non-public enterprises and social benefits, an accurate grasp of legal limits, protect the enthusiasm of entrepreneurs and practitioners in innovation and entrepreneurship, enhancing expectations and confidence. To promote strict enforcement. Strengthening law enforcement procedures, improve law enforcement performance evaluation, strengthening the supervision of law enforcement, exploration and practice of risk control mechanism of law enforcement to further standardize the social security, urban management, road traffic administrative law enforcement, effectively free, extensive law-enforcement and lax enforcement of law enforcement and other issues. To uphold justice. Justice is the last line of Defense to safeguard fairness and justice, to unequivocally support the courts and procuratorates independently exercise level ... Judicial activities were rampant, must not be allowed to run, favors, money, must not be allowed to knowingly violate the miscarriage, violations of the legitimate rights and interests of the masses must not be allowed to abuse of power, to make forest mass can be felt in every judicial cases in equity and justice. Four to deepen grass-roots governance according to lawchain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \visits, like to spend money and buy stop and stable, but disregard the law administration, in accordance with the law, the rulechain do long, and put scale big. To serve the development of enterprises. Duocuo simultaneously improving service efficiency, build better public services platform, enhance work efficiency, initiative to help businesses solve the project procedures, financing loans, issues such as land-use approval, reduce operating costs, business travel light and enhance the confidence to overcome all kinds of difficulties and the Foundation, real entrepreneurial passion play entrepreneurship, enhance the internal vitality and creativity. SI XING an construction, promoting the rule of law, tries to make the transformation of forest development XI General Secretary stressed, to reinvigorate Northeast China, optimized development environment is very important. Environment of rule of law most gather Moss, the most conducive to development. Improving the leading cadres ' using the method of thought and the rule of law Administration work, problem-solving, the ability to promote the development, is the key to promoting the rule of law. The law may affect the efficiency of out of the path of development will be more smooth, won development gains will be sustained, and end with greater efficiency. Leading cadres at all levels should take the lead in respect of law law, abide by, and actively foster Socialist culture, actively promoting the field of multi-level governance according to law, guide the masses and consciously abide by the law, failing to find law, solve the problem by law, in accordance with the law prevail. XXX administration by law of leading cadres do not exist on the rule of law, law enforcement, casual, and vow not to investors, the new scores and other turmoil. These important expositions on my district create good development environment with highly targeted and guidance, especially the General Secretary pointed out that the chaos in my area also exists to varying degrees, some even quite serious. Leading cadres at all levels must improve the development environment of rule of law as a fundamental task, adhere to the problem oriented, solid and solve the problems in the construction of rule of law, to rule the new effect for development environment improvements. To hold \complex issues, often speak of \ A
．该洗衣粉中的生物活性物质是酶
B．实验甲中纱布上蛋白膜消失的原因是洗衣粉中的酶催化蛋白质分解
C．实验乙中纱布上蛋白膜未消失的原因是煮沸使酶变性失活，不能分解蛋白质
D．该实验中的自变量是温度高低，因变量是蛋白膜是否消失，甲是对照组17.D解析：据甲图知道纱布上的蛋白膜加入洗衣粉后，在一定条件下，一段时间后蛋白膜消失，说明洗衣粉中含有能够专一性去掉蛋白膜成分的物质，并由甲、乙对照发现该物质易受温度影响，可判断洗衣粉中的生物活性物质是酶。洗衣粉中的酶在条件适宜的情况下催化蛋白质分解。由于洗衣粉经过煮沸，其中的酶结构破坏、酶失活，不再催化分解蛋白质。该实验中的自变量是温度，因变量是蛋白膜是否消失或消失的时间长短来表示酶是否具有活性，甲是实验组，乙是对照组。
18.下图是应用固定化酵母进行葡萄糖发酵的装置，下列说法中不正确的是 ( A.为使固定化酵母可以反复使用，实验过程一定要在无菌条件下进行 B.加入反应液后应保持活塞1始终打开，活塞2则必须关闭
Grass-roots governance according to law is an important foundation for promoting the construction of rule of law, but also the most basic development environment. Despite my good social order in General, but there are law-abiding consciousness, social order is not standard, regardless of faith, Twining visit visit and other outstanding problems. We want to actively promote the field of multi-level governance according to law, promote the continuous improvement of the social environment. To promote universal compliance. Actively foster the rule of law culture, carry out law publicity and education on honesty and trustworthiness, guide the masses and consciously abide by the law, failing to find method, problem-solving method, method of gradually changing the world, he is not looking for but someone \against violence crime, mafia and serious criminal offences, to protect the legitimate rights and interests of citizens, legal persons, carry out criminal policy of temper justice with mercy, the maximum stimulating social vitality, and earnestly safeguard social harmony and stability. To resolve social conflicts by law. To further smooth channels of reporting social conditions and public opinion, improving regulation, arbitration, as well as dispute resolution mechanisms, implement fully the petitioners ' cases \should want to do work and able to do good, enthusiasm is paramount. Treat party cadres, it is necessary to Strict management, and warm care, enable the broad masses of cadres work hard work, this is two parallel principles. In strict accordance with the standards of good cadres selection and appointment, the real officers, dare officers, those who want to play, good as excellent cadres at all levels of leadership in the past. At present, some leading cadres work initiative is not high, beyond political, idle, lazy governance governance, not as, slow, messy and a phenomenon still exist to varying degrees, affected and restricted economic and social development. We must mobilize the enthusiasm of cadres as an urgent task, adhere to combination of incentives and constraints, and adhere to the strict management and warm care, practical solution \dare to play, good at shows as new. To identify the problem, accurate pulseof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \investors, and must not break faith with the people, promises and the promise to the society. To build a newof law to visit and contribute to make visits to the vexatious visits arrogance. On these issues, we need to have a proper understanding, should take the lead to establish a sense of fear of the law, strengthen the application of the rule of law and the rule of law work of thinking and take up responsibility for the construction of the rule of law. Put learning first. Premise is ignorant of the law, abide by the law. Leading cadres at all levels should take the lead in studying the Constitution and laws and regulations closely related to work assumed by, an accurate grasp of the basic position of our party to address the issue of the rule of law, known for the scale to do things, figure out how to use it right, what things do, what not to do, it can never \by the law makes the right to statutory, as well as the rule of law, develop habits of rule of law, to discard the rule and cannot, must not get overridden by authority, at the right pressure. Should take the lead to abide by. Fulfil its mandate in strict accordance with statutory authority and procedures, no matter what you want to adhere to the law, thought of rule by law and the rule of law to tackle hot issues, effective statutory duty must be, without authorization, is not available. Such as construction areas, some cadres are because there is no procedure is followed by the rules, paid a heavy price, we should learn from them, in strict accordance with the law on bidding and implementation ... Promised to give preferential policies for enterprises, hesitant to cash or cash not at all. Masses feel strongly about these issues, it is necessary to be solved. To accelerate decentralization. Specification list list list of powers, responsibilities and negative, clearance, revocation does not adapt to the current situation of the development of policy documents, especially the right to administrative examination and approval, the cancellation must be canceled, the delegation must be decentralized to achieve zero-wait, one-stop, seamless, improve administrative efficiency and service levels. In particular, the delegation of powers must be caught properly, never able to loft is no answer, no one cares, cause backfire, people work harder. To carry out the campaign. Crack down on law enforcement regulations, uncivil and not as, as undermining economic development, such as environmental problems, pay close attention to the grass-roots level weaknesses, strictly investigate and deal with the grass-roots level corruption and denial of Justice against the interests of the masses, such as corruption and corruption, ruthless people around \companies and investors, and must not break faith with the people, promises and the promise to the society. To build a new)
专题4《酶的研究与应用》单元测试卷(可编辑修改word版)
