针刺跷脉对失眠大鼠视交叉上核内生物钟基因Period1及
Period2的影响
郭保君;余思奕;申治富;胡幼平
【期刊名称】《针刺研究》 【年(卷),期】2017(042)006
【摘要】目的:观察针刺跷脉对失眠大鼠视交叉上核(SCN)内生物钟基因Period(Per)1及Per 2表达水平的影响,探索其内在机制.方法:SD大鼠随机分为空白组、模型组、针刺组,每组10只.采用腹腔注射对氯苯丙氨酸混悬液1 mL/100 g复制失眠模型.针刺组针刺“申脉”“照海”,15 min/次,1次/d,连续7d.观察大鼠昼夜节律的变化,real time-PCR技术检测SCN内Per 1及Per2 mRNA表达水平.结果:与空白组比较,模型组大鼠每日休息期活动量显著增加,SCN内Per1及Per 2 mRNA表达水平显著降低(P＜0.05).与模型组比较,针刺组大鼠休息期活动量显著减少,SCN内Per 1及Per 2 mRNA表达水平显著上升(P＜0.05).结论:针刺“申脉”“照海”可提高SCN内Per 1、Per 2 mRNA表达,减少失眠大鼠休息期活动量,从而达到治疗失眠的目的.%Objective To investigate the influence and mechanism of acupuncture at the points in Heel Vessel for the circadian clock genes of Period (Per) 1 and Per 2 mRNAs in the suprachiasmatic nucleus (SCN) in insomnia rats.Methods Thirty male SD rats were randomly divided into blank,model,acupuncture groups,10 rats in each group.Insomnia model was established by intraperitoneal injection of PCPA (suspension,1 mL/100 g).Acupuncture at \(BL 62) and \(KI 6) was
针刺跷脉对失眠大鼠视交叉上核内生物钟基因Period1及Period2的影响
