TGF-β 1-promoted epithelial-to-mesenchymal transformation and cell adhesion contribute to

TGF-β 1-promoted epithelial-to-mesenchymal transformation and cell adhesion contribute to TGF-β1-enhanced cell migration in SMMC-7721

cells

SHU BING ZHANG;RUO LAN QIAN;JIAN HUANG;HUI ZHAO;YI ZHANG;CHUN HUI HOU;XIAO DONG CHENG;CHU JIANG;MIN QIAN LI;JUN HU

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2003(013)005

【摘要】Transforming growth factor-bi (TGF-β1), a multi-function polypeptide, is a double-edged sword in cancer.For some tumor cells, TGF-β1 is a potent growth inhibitor and apoptosis inducer. More commonly, TGF-β1 loses its growth-inhibitory and apoptosis-inducing effects, but stimulates the metastatic capacity of tumor cells. It is currently little known about TGF-β1-promoted cell migration in hepatocellular carcinoma (HCC) cells, let alone its mechanism. In this study, we found that TGF-β1 lost its tumor-suppressive effects, but significantly stimulated cell migration in SMMC-7721 human HCC cells. By FACS and Western blot analysis, we observed that TGF-β1 enhanced the expression of α5β1 integrin obviously, and subsequently stimulated cell adhesion onto fibronectin (Fn). Furthermore, we observed that TGF-β1 could also promote SMMC-7721 cells adhesion onto laminin (Ln).Our data also provided evidences that TGF-β1 induced epithelial-to-