单核细胞趋化因子在狼疮性肾炎患者肾间质单核细胞浸润
及损伤中的作用
戴春笋;刘志红;周虹;黎磊石
【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2001(114)008 【摘要】Objective
To investigate the pattern of monocyte
chemoattractant prolein-1 (MCP-1) distribution in the renal interstitium and evaluate its pathogenic role in tubulo-interstitial lesions in patients with lupus nephritis, the distribution of MCP-1 in renal tissue was observed.rnMethods Eighteen female patients with biopsy-proven lupus
nephritis
were
enrolled
in
this
study.
No
intensive
immunosuppresive therapy was used in these patients during the 3 months prior to renal biopsy. The distribution of MCP-1, infiltration of CD68+ (macrophage/monocyte), CD4+ and CD8+ cells in the tubulo-interstitium of patients with lupus nephritis was detected using immunohistochemical
staining
with
specific
antibodies.
Renal
specimens from patients with minimal change glomerulonephritis were used as controls. rnResults MCP-1 protein was widely distributed in the renal tissue of patients with lupus nephritis, mainly located at the baso-lateral surface of tubular epithelial cells (16/18 biopsies), and on the wall of interstitial blood vessels (9/18 biopsies). In contrast, tubular MCP-1 staining was weak and rare in renal tissue from controls (7.4±6.2%
vs 26.7±22.8%, P<0.01). Tubulo-interstitial infiltration of CD68+, CD4+ and CD8+ cells was markedly increased in patients with lupus nephritis as compared to controls. The tubular expression of MCP-1 was strongly associated with the amount of CD68+ cell infiltration in the interstitium (r=0.5420, P<0.05) and the extent of interstitial fibrosis. There was no correlation between MCP-1 production in tubules and the degree of urinary protein excretion in patients with lupus nephritis (r=0.0547, P>0.05).rnConclusions The expression of MCP-1 in the renal tubules and vascular wall was markedly increased in patients with lupus nephritis. The overproduction of MCP-1 in renal tissue may contribute to monocyte recruitment in the interstitium and thus result in tubulo-interstitial damage in lupus nephritis.%目的观察狼疮性肾炎（LN）患者肾组织中单核细胞趋化因子（MCP-1）的分布及其与肾间质单核细胞浸润和间质病变的关系，探讨其在LN患者肾小管间质病变中的作用。rn方法采用PAP四层法对18例LN患者肾组织MCP-1，CD68+，CD4+，及CD8+进行免疫组化染色。同时取8例微小病变型肾病患者作为对照。rn结果 18例患者肾组织中均有不同程度的MCP-1染色阳性。其主要位于肾小管基侧膜（16/18例）及间质小血管壁（9/18例）上。对照组MCP-1阳性的小管明显低于狼疮性肾炎患者（7.4±6.2% vs 26.7±22.8%, P<0.01）。LN患者肾间质中CD4+，CD8+及CD68+细胞数明显高于对照。其MCP-1阳性小管数与间质CD68+细胞浸润及间质病变的程度明显相关。而与CD4+，CD8+细胞浸润程度及患者尿蛋白的多少无相关性。rn结论 LN患者肾小管MCP-1表达增加可能是导
致间质单核细胞浸润及肾小管间质损伤的一个重要原因。 【总页数】5页(864-868)
【关键词】单核细胞趋化因子;狼疮性肾炎;小管间质病变 【作者】戴春笋;刘志红;周虹;黎磊石
【作者单位】南京大学医学院临床学院肾脏科南京 210002;南京大学医学院临床学院肾脏科南京 210002;南京大学医学院临床学院肾脏科南京 210002;南京大学医学院临床学院肾脏科南京 210002 【正文语种】中文 【中图分类】R69 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-medical-journal_thesis/0201255014320.html 【相关文献】
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单核细胞趋化因子在狼疮性肾炎患者肾间质单核细胞浸润及损伤中的作用
