YADE，一种新的棉花gDNA及cDNA的PCR步移方法
Yue-hua XIAO;Ming LUO;Shang-yong ZHENG;Wei-guo FANG;Lei HOU;Yan PEI
【期刊名称】《棉花学报》 【年(卷),期】2002(014)0Z1
【摘要】@@ The isolation of flanking sequences is a crucial step in the study of gene cloning and expression.On the basis of Y-shaped adaptor (Prashar and Weissman, 1996), we designed a new method which could be used to amplify the flanking region from both genomic DNA and cDNA. This method was designated as Y-shaped adaptor dependent extesion (YADE). The schematic of this method is showed in fig. 1. 【总页数】1页(67) 【关键词】
【作者】Yue-hua XIAO;Ming LUO;Shang-yong ZHENG;Wei-guo FANG;Lei HOU;Yan PEI
【作者单位】Biotechnology Research Center, Southwest Agricultural University, Chongqing 400716, China;Biotechnology Research Center, Southwest
Agricultural
University,
Chongqing
400716,
China;Biotechnology Research Center, Southwest Agricultural University, Chongqing 400716, China;Biotechnology Research Center, Southwest Agricultural University,
Chongqing 400716,
China;Biotechnology
Research Center, Southwest Agricultural University, Chongqing 400716, China;Biotechnology Research Center, Southwest Agricultural University,
YADE,一种新的棉花gDNA及cDNA的PCR步移方法
