二烯丙基三硫化物对人神经母细胞瘤细胞SK-N-SH生长的
抑制作用
曾纪晓;夏慧敏;朱德力;邹焱;余家康
【期刊名称】《中华实验外科杂志》 【年(卷),期】2013(030)003
【摘要】目的 探讨二烯丙基三硫化物(DATS)对人神经母细胞瘤细胞SK-N-SH生长的影响及相关机制.方法 光镜下观察不同浓度DATS对SK-N-SH细胞生长的影响；细胞分为对照组、DATSⅠ组(10 μg/L)和DATSⅡ组(40μg/L),DATS作用24、48 h后,流式细胞仪测定细胞凋亡；作用72 h后,荧光定量聚合酶链反应(FQ-PCR)法测定DATS对SK-N-SH细胞血管内皮生长因子(VEGF)mRNA、细胞间黏附分子-1(ICAM-1) mRNA表达的影响；酶联免疫吸附试验(EHSA)法测定细胞培养上清液VEGF、ICAM-1浓度.结果 不同浓度的DATS均能抑制SK-N-SH细胞的生长,小剂量DATS能诱导细胞凋亡；对照组、DATSⅠ组和Ⅱ组VEGF mRNA、ICAM-1 mRNA表达量分别为5.97±1.31、4.21±1.06、3.35±0.77(P ＜0.05)和4.41±0.94、3.22 ±1.17、2.69±0.73 (P＜0.05)；对照组、DATS Ⅰ组和Ⅱ组SK-N-SH细胞培养上清液中VEGF、ICAM-1分别为(72.1 ±26.9)、(51.6±17.3)、(40.3±11.8) μg/L(P ＜0.05)和(311.6±83.7)、(274.2 ±71.0)、(200.6 ±42.9) ng/L(P＜0.05).结论 DATS在体外可抑制神经母细胞瘤细胞SK-N-SH的生长,DATS能诱导SK-N-SH细胞凋亡,下调其VEGF mRNA、ICAM-1 mRNA的转录和蛋白表达.%Objective To investigate the efficacy and the possible mechanism of human neuroblastoma cell line SK-N-SH induced by diallyl trisulfide (DATS) in vitro.Methods Flow
cytometry was applyed to test the apoptosis rate of human neuroblastoma cell lines SK-N-SH after DATS was used 24 h and 48 h.SK-N-SH cells were randomly divided into 3 groups:the control group,the DATS group Ⅰ (DATS10 μg/L) and group Ⅱ (DATS 40 μg/L),the role of DATS on the growth of SK-N-SH cells was evaluated after 72 h.The expression of vascular endothelial growth factor (VEGF) mRNA and intercellular adhesion molecule-1 (ICAM-1) mRNA of SK-N-SH cells were detected by fluorescent quantitation ploymerase chain reaction (FQ-PCR).The VEGF and ICAM-1 concentration of supematants were measured by enzyme linked immunosorbent assay (ELISA).Results DATS inhibited the growth of SK-N-SH cells in vitro,small dose of DATS induced apoptosis of SK-N-SH cells (P ＜ 0.05).The expression of VEGF mRNA in the control group,the DATS group I and DATS group Ⅱ was (5.97 ±1.31),(4.21 ±1.06) and (3.35 ±0.77) (P＜0.05),the expression of ICAM-1 mRNA was (4.41±0.94),(3.22 ±1.17) and (2.69 ±0.73) (P ＜ 0.05).The VEGF concentration of culture supernatants of SK-N-SH cells was (72.1 ±26.9),(51.6 ±17.3) and (40.3 ± 11.8) μg/L in the 3 groups (P ＜0.05),and the ICAM-1 concentration was (311.6±83.7),(274.2 ±71.0) and (200.6±42.9) ng/L (P＜0.05).Conclusion DATS can inhibit the growth of human neuroblastoma cell lines SK-N-SH in vitro,which may caused by inducing apoptosis and down-regulating the expression of VEGF and ICAM-1.
【总页数】3页(533-535)
【关键词】二烯丙基三硫化物;肿瘤;神经母细胞瘤;血管内皮生长因子;细胞间黏附分子
【作者】曾纪晓;夏慧敏;朱德力;邹焱;余家康
【作者单位】510120 广州市妇女儿童医疗中心普通外科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-experimental-surgery_thesis/0201234777565.html 【相关文献】
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二烯丙基三硫化物对人神经母细胞瘤细胞SK-N-SH生长的抑制作用
