NF-κB信号通路在类风湿关节炎患者甲氨蝶呤耐药机制中
的作用
姚血明;梁爽;马武开;宁乔怡;钟琴;侯雷;黄颖;唐芳
【期刊名称】《山东医药》 【年(卷),期】2017(057)014
【摘要】Objective To investigate the role of nuclear factor-κB (NF-κB) signaling pathway in the mechanisms of methotrexate (MTX) resistance of patients with rheumatoid arthritis (RA).Methods Totally 120 RA patients taking MTX were selected and were divided into MTX sensitive group and MTX resistant group according to their joint disease activity (DAS28) score,and another 20 healthy volunteers were chosen as the normal control group.ELISA was used to detect the levels of NF-κB,inhibitory protein IκBα (IκBα),TNF-α and IL-1β in serum of each group.The expression of multidrug resistance gene 1 (MDR1),TNF-α and IL-1β mRNA in the MTX sensitive group and MTX resistant group was detected by fluorescence PCR.Flow cytometry was used to detect the expression and function of P glycoprotein (P-gp) in the peripheral blood mononuclear cells (PBMCs).Results The levels of NF-κB,TNF-α and IL-1β in the MTX sensitive group and MTX resistant group were higher,and the IκBα levels were lower than those of the normal control group (all P<0.05).The levels of NF-κB,TNF-α and IL-1β in the MTX resistant group were higher than those in the MTX sensitive group,and the level of IκBα
NF-κB信号通路在类风湿关节炎患者甲氨蝶呤耐药机制中的作用
