Neutrophil extracellular traps in the intestinal mucosa of Eimeria-infected animals

Neutrophil extracellular traps in the intestinal

mucosa of Eimeria-infected animals

Tamara Mu?oz-Caro;Liliana Machado Ribeiro da Silva;Zaída Rentería-Solis;Anja Taubert;Carlos Hermosilla;

【期刊名称】《亚太热带生物医学杂志：英文版》 【年(卷),期】2016(000)004

【摘要】Objective:To investigate the presence of neutrophil extracellular traps(NETs) in vivo by analysing intestinal sections from experimentally Eimeria

bovis-and

naturally

Eimeria

arloingi-infected

animals.Methods:Intestinal samples of Eimeria arloingi-and Eimeria bovis-infected animals were analysed by using immunohistochemical and

fluorescence

approach

by

using

monoclonal

antibodies.Results:Classical NET components were confirmed by co-localization of extracellular DNA being decorated with neutrophil elastase and histones in Eimeria-infected tissue samples.Here,extrusion of NETs was exclusively detected in intestinal polymorphonuclear neutrophils infiltrating Eimeria-infected sites.In vivo NETs were either found in close proximity or in direct contact to different Eimeria stages suggesting a stage-independent process.NETs were also found within the gut lumen driven by polymorphonuclear neutrophils that were contacting released oocysts.Conclusions:We postulate that NETs might play an important role in innate defence reactions in coccidiosis