局部应用紫杉醇抑制兔气管损伤后瘢痕组织形成的初步研究

局部应用紫杉醇抑制兔气管损伤后瘢痕组织形成的初步研

究

王利换;张杰;陈楠;张莹莹;徐敏;岳英明

【期刊名称】《中华结核和呼吸杂志》 【年(卷),期】2013(036)003

【摘要】Objective To investigate the effect of local application of paclitaxel on airway scar formation after airway injury in rabbits.Methods Forty New Zealand rabbits were randomly divided into four groups,negative control group (n =10),saline control group (n =10),group Ⅰ (n =10),group Ⅱ (n =10).All rabbits received tracheotomy.In negative control group,the specimens were harvested for histological examination and immunohistochemical analysis immediately after tracheotomy; in other three groups,rabbits received airway injury after tracheotomy.In group Ⅰ and group Ⅱ,0.4 mg/ml and 1.0 mg/ml paclitaxel was applied locally in injured airway segment for 3 minutes after airway injury.The normal saline was used in control group for 3 minutes.The animals were killed in 21 days after operation.The specimens

were

harvested

for

histological

examination

and

immunohistochemical analysis.Transmission electron microscopy was employed to observe the ultrastructure of paclitaxel-induced apoptotic cells.Results The degree of stenosis in group Ⅰ and group Ⅱ were significantly decreased compared to those in saline control group