Phot2-regulated relocation of NPH3 mediates phototropic response to high-intensity blue li

Phot2-regulated relocation of NPH3 mediates phototropic response to high-intensity blue light in

Arabidopsis thaliana

Xiang Zhao;Qingping Zhao;Chunye Xu;Jin Wang;Jindong Zhu;Baoshuan Shang;Xiao Zhang

【期刊名称】《植物学报（英文版）》 【年(卷),期】2018(060)007

【摘要】Two redundant blue-light receptors,known as phototropins (phot1

and

phot2),influence

a

variety

of

physiological

responses,including phototropism,chloroplast positioning,and stomatal opening in Arabidopsis thaliana.Whereas phot1 functions in both low-and high-intensity blue light (HBL),phot2 functions primarily in HBL.Here,we aimed to elucidate phot2-specific functions by screening for HBL-insensitive mutants among mutagenized Arabidopsis phot1 mutants.One of the resulting phot2 signaling associated (p2sa) double mutants,phot1 p2sa2,exhibited phototropic defects that could be restored by constitutively expressing NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3),indicating that P2SA2 was allelic to NPH3.It was observed that NPH3-GFP signal mainly localized to and clustered on the plasma membrane in darkness.This NPH3 clustering on the plasma membrane was not affected by mutations in genes encoding proteins that interact with NPH3,including PHOT1,PHOT2 and ROOT PHOTOTROPISM 2

(RPT2).However,the H BL irradiation-mediated release of NPH3 proteins into the cytoplasm was inhibited in phot1 mutants and enhanced in phot2

and

rpt2-2

mutants.Furthermore,HBL-induced

hypocotyl

phototropism was enhanced in phot1 mutants and inhibited in the phot2 and rpt2-2 mutants.Our findings indicate that phot1 regulates the dissociation of NPH3 from the plasma membrane,whereas phot2 mediates the stabilization and relocation of NPH3 to the plasma membrane to acclimate to HBL. 【总页数】16页(562-577) 【关键词】

【作者】Xiang Zhao;Qingping Zhao;Chunye Xu;Jin Wang;Jindong Zhu;Baoshuan Shang;Xiao Zhang

【作者单位】Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China;Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China;Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China;Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China;Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng

475004, China;Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China;Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_journal-integrative-plant-biology_thesis/0201270261011.html 【相关文献】

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